Assay Detail
Functional
CHEMBL1654781
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antimycobacterial activity against Mycobacterium smegmatis grown in Luria-Bertani broth medium assessed as growth inhibition in mid-logarithmic phase by GFP-based fluorescence assay
48
Total Activities
24
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Mycolicibacterium smegmatis |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Target
Mycolicibacterium smegmatis (CHEMBL613088) ↗| Type | ORGANISM |
| Organism | Mycolicibacterium smegmatis |
Publication
Modifying culture conditions in chemical library screening identifies alternative inhibitors of mycobacteria.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 24 | 5.16 | 6.12 |
| MIC | 24 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1200699 | DOXYCYCLINE | 4.0 | 2 | 6.12 |
| CHEMBL1256686 | CALCIMYCIN | 2.0 | 2 | 5.98 |
| CHEMBL1591 | DEMECLOCYCLINE | 4.0 | 2 | 5.73 |
| CHEMBL4 | OFLOXACIN | 4.0 | 2 | 5.69 |
| CHEMBL262777 | VANCOMYCIN | 4.0 | 2 | 5.50 |
| CHEMBL1117 | IDARUBICIN | 4.0 | 2 | 5.44 |
| CHEMBL1315457 | — | — | 2 | 5.40 |
| CHEMBL561 | LOMEFLOXACIN | 4.0 | 2 | 5.36 |
| CHEMBL104 | CLOTRIMAZOLE | 4.0 | 2 | 5.18 |
| CHEMBL1434 | MINOCYCLINE | 4.0 | 2 | 5.17 |
| CHEMBL365739 | DIPHENYLENEIODONIUM | — | 2 | 5.17 |
| CHEMBL1448 | NICLOSAMIDE | 4.0 | 2 | 5.16 |
| CHEMBL575867 | 4-CHLOROMERCURIBENZOIC ACID | — | 2 | 5.15 |
| CHEMBL1650592 | — | — | 2 | 5.09 |
| CHEMBL422 | TRIFLUOPERAZINE | 4.0 | 2 | 4.91 |
| CHEMBL1208858 | CALMIDAZOLIUM CHLORIDE | — | 2 | 4.88 |
| CHEMBL500996 | METHOCTRAMINE HYDROCHLORIDE | — | 2 | 4.80 |
| CHEMBL58 | MITOXANTRONE | 4.0 | 2 | 4.66 |
| CHEMBL55 | PENTAMIDINE | 4.0 | 2 | 4.63 |
| CHEMBL1079460 | — | — | 2 | 4.60 |
| CHEMBL270299 | BAY-11-7085 | — | 2 | 4.51 |
| CHEMBL415879 | PHENANTHROLINE | — | 2 | 4.38 |
| CHEMBL62382 | METHYLSELENOCYSTEINE | 1.0 | 2 | - |
| CHEMBL3216717 | — | — | 2 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1200699 | DOXYCYCLINE | IC50 | = | 760.0 | nM | 6.12 |
| CHEMBL1256686 | CALCIMYCIN | IC50 | = | 1040.0 | nM | 5.98 |
| CHEMBL1591 | DEMECLOCYCLINE | IC50 | = | 1880.0 | nM | 5.73 |
| CHEMBL4 | OFLOXACIN | IC50 | = | 2030.0 | nM | 5.69 |
| CHEMBL262777 | VANCOMYCIN | IC50 | = | 3180.0 | nM | 5.50 |
| CHEMBL1117 | IDARUBICIN | IC50 | = | 3610.0 | nM | 5.44 |
| CHEMBL1315457 | — | IC50 | = | 3950.0 | nM | 5.40 |
| CHEMBL561 | LOMEFLOXACIN | IC50 | = | 4330.0 | nM | 5.36 |
| CHEMBL104 | CLOTRIMAZOLE | IC50 | = | 6540.0 | nM | 5.18 |
| CHEMBL1434 | MINOCYCLINE | IC50 | = | 6770.0 | nM | 5.17 |
| CHEMBL365739 | DIPHENYLENEIODONIUM | IC50 | = | 6770.0 | nM | 5.17 |
| CHEMBL1448 | NICLOSAMIDE | IC50 | = | 6850.0 | nM | 5.16 |
| CHEMBL575867 | 4-CHLOROMERCURIBENZOIC ACID | IC50 | = | 7070.0 | nM | 5.15 |
| CHEMBL1650592 | — | IC50 | = | 8150.0 | nM | 5.09 |
| CHEMBL422 | TRIFLUOPERAZINE | IC50 | = | 12260.0 | nM | 4.91 |
| CHEMBL1208858 | CALMIDAZOLIUM CHLORIDE | IC50 | = | 13200.0 | nM | 4.88 |
| CHEMBL500996 | METHOCTRAMINE HYDROCHLORIDE | IC50 | = | 15900.0 | nM | 4.80 |
| CHEMBL58 | MITOXANTRONE | IC50 | = | 21840.0 | nM | 4.66 |
| CHEMBL55 | PENTAMIDINE | IC50 | = | 23530.0 | nM | 4.63 |
| CHEMBL1079460 | — | IC50 | = | 25110.0 | nM | 4.60 |
| CHEMBL270299 | BAY-11-7085 | IC50 | = | 30600.0 | nM | 4.51 |
| CHEMBL415879 | PHENANTHROLINE | IC50 | = | 41640.0 | nM | 4.38 |
| CHEMBL1434 | MINOCYCLINE | MIC | = | 12500.0 | nM | - |
| CHEMBL58 | MITOXANTRONE | MIC | = | 50000.0 | nM | - |
| CHEMBL1448 | NICLOSAMIDE | MIC | = | 12500.0 | nM | - |
| CHEMBL4 | OFLOXACIN | MIC | = | 3125.0 | nM | - |
| CHEMBL55 | PENTAMIDINE | MIC | = | 50000.0 | nM | - |
| CHEMBL415879 | PHENANTHROLINE | MIC | = | 50000.0 | nM | - |
| CHEMBL62382 | METHYLSELENOCYSTEINE | MIC | > | 50000.0 | nM | - |
| CHEMBL422 | TRIFLUOPERAZINE | MIC | = | 50000.0 | nM | - |
| CHEMBL3216717 | — | MIC | = | 50000.0 | nM | - |
| CHEMBL262777 | VANCOMYCIN | MIC | = | 12500.0 | nM | - |
| CHEMBL1650592 | — | MIC | = | 50000.0 | nM | - |
| CHEMBL62382 | METHYLSELENOCYSTEINE | IC50 | > | 50000.0 | nM | - |
| CHEMBL3216717 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL270299 | BAY-11-7085 | MIC | = | 50000.0 | nM | - |
| CHEMBL1208858 | CALMIDAZOLIUM CHLORIDE | MIC | = | 12500.0 | nM | - |
| CHEMBL1256686 | CALCIMYCIN | MIC | = | 3125.0 | nM | - |
| CHEMBL104 | CLOTRIMAZOLE | MIC | = | 12500.0 | nM | - |
| CHEMBL1591 | DEMECLOCYCLINE | MIC | = | 3125.0 | nM | - |
| CHEMBL1315457 | — | MIC | = | 6250.0 | nM | - |
| CHEMBL365739 | DIPHENYLENEIODONIUM | MIC | = | 6250.0 | nM | - |
| CHEMBL1200699 | DOXYCYCLINE | MIC | = | 1560.0 | nM | - |
| CHEMBL1117 | IDARUBICIN | MIC | = | 12500.0 | nM | - |
| CHEMBL561 | LOMEFLOXACIN | MIC | = | 12500.0 | nM | - |
| CHEMBL1079460 | — | MIC | = | 50000.0 | nM | - |
| CHEMBL500996 | METHOCTRAMINE HYDROCHLORIDE | MIC | = | 12500.0 | nM | - |
| CHEMBL575867 | 4-CHLOROMERCURIBENZOIC ACID | MIC | = | 25000.0 | nM | - |