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Assay Detail

CHEMBL2157385

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Reversible inhibition of human recombinant FAAH assessed as hydrolysis of [3H]-AEA after 10 mins by liquid scintillation counting
12
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty-acid amide hydrolase 1 (CHEMBL2243)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

SAR and LC/MS studies of β-lactamic inhibitors of human fatty acid amide hydrolase (hFAAH): evidence of a nonhydrolytic process.
Feledziak M, Muccioli GG, Lambert DM, Marchand-Brynaert J.
J Med Chem (2011) 54 : 6812 -6823
PubMed 21899370 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 5.97 8.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL177577 1 8.33
CHEMBL2151432 2 8.30
CHEMBL560590 1 7.48
CHEMBL2151435 2 5.54
CHEMBL2151043 2 4.08
CHEMBL2151433 2 4.05
CHEMBL2151434 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL177577 IC50 = 4.7 nM 8.33
CHEMBL2151432 IC50 = 5.0 nM 8.30
CHEMBL2151432 IC50 = 5.37 nM 8.27
CHEMBL560590 IC50 = 33.0 nM 7.48
CHEMBL2151435 IC50 = 2900.0 nM 5.54
CHEMBL2151435 IC50 = 2884.03 nM 5.54
CHEMBL2151043 IC50 = 82800.0 nM 4.08
CHEMBL2151043 IC50 = 83176.38 nM 4.08
CHEMBL2151433 IC50 = 88600.0 nM 4.05
CHEMBL2151433 IC50 = 89125.09 nM 4.05
CHEMBL2151434 IC50 > 1000000.0 nM -
CHEMBL2151434 IC50 > 316227.77 nM -