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Assay Detail

CHEMBL2320298

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human MATE1-mediated [14]-metformin uptake expressed in polarized MDCK2 cells after 5 mins by liquid scintillation counting analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDCK-II
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Multidrug and toxin extrusion protein 1 (CHEMBL1743126)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent, selective multidrug and toxin extrusion transporter 1 (MATE1, SLC47A1) inhibitors through prescription drug profiling and computational modeling.
Wittwer MB, Zur AA, Khuri N, Kido Y, Kosaka A, Zhang X, Morrissey KM, Sali A, Huang Y, Giacomini KM.
J Med Chem (2013) 56 : 781 -795
PubMed 23241029 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.13 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL941 IMATINIB 4.0 1 7.40
CHEMBL58 MITOXANTRONE 4.0 1 6.72
CHEMBL55 PENTAMIDINE 4.0 1 6.39
CHEMBL360861 NIFEKALANT 3.0 1 6.19
CHEMBL5483011 INDINAVIR 3.0 1 5.72
CHEMBL163 RITONAVIR 4.0 1 5.68
CHEMBL1502 PANTOPRAZOLE 4.0 1 5.55
CHEMBL481 IRINOTECAN 4.0 1 5.37
CHEMBL46 ONDANSETRON 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL941 IMATINIB IC50 = 40.0 nM 7.40
CHEMBL58 MITOXANTRONE IC50 = 190.0 nM 6.72
CHEMBL55 PENTAMIDINE IC50 = 410.0 nM 6.39
CHEMBL360861 NIFEKALANT IC50 = 650.0 nM 6.19
CHEMBL5483011 INDINAVIR IC50 = 1900.0 nM 5.72
CHEMBL163 RITONAVIR IC50 = 2100.0 nM 5.68
CHEMBL1502 PANTOPRAZOLE IC50 = 2800.0 nM 5.55
CHEMBL481 IRINOTECAN IC50 = 4300.0 nM 5.37
CHEMBL46 ONDANSETRON IC50 < 10.0 nM -