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Assay Detail

CHEMBL2345299

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]dihydroalprenolol from human wild type beta1 adrenergic receptor expressed in HEK293 cell membranes after 90 mins by liquid scintillation counting analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-1 adrenergic receptor (CHEMBL213)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Biophysical fragment screening of the β1-adrenergic receptor: identification of high affinity arylpiperazine leads using structure-based drug design.
Christopher JA, Brown J, Doré AS, Errey JC, Koglin M, Marshall FH, Myszka DG, Rich RL, Tate CG, Tehan B, Warne T, Congreve M.
J Med Chem (2013) 56 : 3446 -3455
PubMed 23517028 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 6.25 7.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL200234 1 7.17
CHEMBL2335158 1 7.07
CHEMBL1424807 1 6.97
CHEMBL2335166 1 6.70
CHEMBL2335159 1 6.67
CHEMBL1559535 1 6.65
CHEMBL2335164 1 6.60
CHEMBL2335161 1 6.27
CHEMBL2335167 1 6.17
CHEMBL2335160 1 6.13
CHEMBL2335162 1 5.90
CHEMBL9434 PHENYLPIPERAZINE 1 5.87
CHEMBL2335163 1 5.80
CHEMBL2335165 1 5.80
CHEMBL2335156 1 5.63
CHEMBL2335157 1 5.60
CHEMBL18772 QUIPAZINE 2.0 1 5.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL200234 Ki = 67.61 nM 7.17
CHEMBL2335158 Ki = 85.11 nM 7.07
CHEMBL1424807 Ki = 107.15 nM 6.97
CHEMBL2335166 Ki = 199.53 nM 6.70
CHEMBL2335159 Ki = 213.8 nM 6.67
CHEMBL1559535 Ki = 223.87 nM 6.65
CHEMBL2335164 Ki = 251.19 nM 6.60
CHEMBL2335161 Ki = 537.03 nM 6.27
CHEMBL2335167 Ki = 676.08 nM 6.17
CHEMBL2335160 Ki = 741.31 nM 6.13
CHEMBL2335162 Ki = 1258.93 nM 5.90
CHEMBL9434 PHENYLPIPERAZINE Ki = 1348.96 nM 5.87
CHEMBL2335163 Ki = 1584.89 nM 5.80
CHEMBL2335165 Ki = 1584.89 nM 5.80
CHEMBL2335156 Ki = 2344.23 nM 5.63
CHEMBL2335157 Ki = 2511.89 nM 5.60
CHEMBL18772 QUIPAZINE Ki = 6309.57 nM 5.20