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Assay Detail

CHEMBL2390167

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ORAl1/2/3 in HEK293 cells assessed as inhibition of Cd2+ influx after 10 mins by FLIPR assay in presence of thapsigargin
36
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

ORAI 1/2/3 (CHEMBL3038476)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Design, synthesis and pharmacological characterization of analogs of 2-aminoethyl diphenylborinate (2-APB), a known store-operated calcium channel blocker, for inhibition of TRPV6-mediated calcium transport.
Hofer A, Kovacs G, Zappatini A, Leuenberger M, Hediger MA, Lochner M.
Bioorg Med Chem (2013) 21 : 3202 -3213
PubMed 23602525 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 36 5.02 5.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2386000 1 5.66
CHEMBL2386180 1 5.62
CHEMBL2386179 1 5.57
CHEMBL2386172 1 5.50
CHEMBL2386185 1 5.31
CHEMBL2385996 1 5.29
CHEMBL2386174 1 5.23
CHEMBL2386184 1 5.19
CHEMBL2386186 1 5.14
CHEMBL2386009 1 5.13
CHEMBL2386181 1 5.11
CHEMBL2385999 1 5.09
CHEMBL81556 DIPHENYL BORINIC ACID 1 5.06
CHEMBL2386171 1 5.02
CHEMBL2386004 1 5.01
CHEMBL2386177 1 5.01
CHEMBL2386178 1 5.00
CHEMBL2385998 1 5.00
CHEMBL2386001 1 4.99
CHEMBL2385997 1 4.99
CHEMBL2386011 1 4.98
CHEMBL2386003 1 4.96
CHEMBL2386010 1 4.93
CHEMBL2385995 1 4.92
CHEMBL2386188 1 4.87
CHEMBL2386005 1 4.83
CHEMBL2386173 1 4.77
CHEMBL2386183 1 4.60
CHEMBL2386008 1 4.59
CHEMBL2386175 1 4.59

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2386000 IC50 = 2200.0 nM 5.66
CHEMBL2386180 IC50 = 2400.0 nM 5.62
CHEMBL2386179 IC50 = 2700.0 nM 5.57
CHEMBL2386172 IC50 = 3200.0 nM 5.50
CHEMBL2386185 IC50 = 4900.0 nM 5.31
CHEMBL2385996 IC50 = 5100.0 nM 5.29
CHEMBL2386174 IC50 = 5900.0 nM 5.23
CHEMBL2386184 IC50 = 6400.0 nM 5.19
CHEMBL2386186 IC50 = 7200.0 nM 5.14
CHEMBL2386009 IC50 = 7400.0 nM 5.13
CHEMBL2386181 IC50 = 7700.0 nM 5.11
CHEMBL2385999 IC50 = 8200.0 nM 5.09
CHEMBL81556 DIPHENYL BORINIC ACID IC50 = 8700.0 nM 5.06
CHEMBL2386171 IC50 = 9500.0 nM 5.02
CHEMBL2386177 IC50 = 9800.0 nM 5.01
CHEMBL2386004 IC50 = 9800.0 nM 5.01
CHEMBL2386178 IC50 = 9900.0 nM 5.00
CHEMBL2385998 IC50 = 10100.0 nM 5.00
CHEMBL2386001 IC50 = 10200.0 nM 4.99
CHEMBL2385997 IC50 = 10200.0 nM 4.99
CHEMBL2386011 IC50 = 10500.0 nM 4.98
CHEMBL2386003 IC50 = 11000.0 nM 4.96
CHEMBL2386010 IC50 = 11800.0 nM 4.93
CHEMBL2385995 IC50 = 11900.0 nM 4.92
CHEMBL2386188 IC50 = 13400.0 nM 4.87
CHEMBL2386005 IC50 = 14700.0 nM 4.83
CHEMBL2386173 IC50 = 17100.0 nM 4.77
CHEMBL2386183 IC50 = 25200.0 nM 4.60
CHEMBL2386008 IC50 = 25900.0 nM 4.59
CHEMBL2386175 IC50 = 25800.0 nM 4.59
CHEMBL2386176 IC50 = 36400.0 nM 4.44
CHEMBL2386006 IC50 = 44600.0 nM 4.35
CHEMBL2386007 IC50 = 184200.0 nM -
CHEMBL257679 METHANE BORONIC ACID IC50 > 500000.0 nM -
CHEMBL2386190 DIPHENYLMETHANOL IC50 > 500000.0 nM -
CHEMBL657 DIPHENHYDRAMINE IC50 > 500000.0 nM -