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Assay Detail

CHEMBL2424067

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 5-(3-(3-(6-amino-8-(6-iodobenzo[d][1,3]dioxol-5-ylthio)-9H-purin-9-yl)propyl)thioureido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid from recombinant HSP90beta (unknown origin) after 24 hrs by fluorescence polarization assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Heat shock protein HSP 90-beta (CHEMBL4303)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Experimental and structural testing module to analyze paralogue-specificity and affinity in the Hsp90 inhibitors series.
Taldone T, Patel PD, Patel M, Patel HJ, Evans CE, Rodina A, Ochiana S, Shah SK, Uddin M, Gewirth D, Chiosis G.
J Med Chem (2013) 56 : 6803 -6818
PubMed 23965125 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.30 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2103879 GANETESPIB 3.0 1 8.30
CHEMBL252164 LUMINESPIB 2.0 1 7.80
CHEMBL467399 BIIB021 2.0 1 7.77
CHEMBL278315 GELDANAMYCIN 2.0 1 7.66
CHEMBL560895 SNX-2112 -1.0 1 7.60
CHEMBL2419346 CUDC-305 1.0 1 7.42
CHEMBL200102 PU-H71 2.0 1 7.38
CHEMBL109480 TANESPIMYCIN 3.0 1 7.35
CHEMBL14830 ADENOSINE DIPHOSPHATE 0.5 1 4.38
CHEMBL14249 ADENOSINE TRIPHOSPHATE 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2103879 GANETESPIB IC50 = 5.0 nM 8.30
CHEMBL252164 LUMINESPIB IC50 = 16.0 nM 7.80
CHEMBL467399 BIIB021 IC50 = 17.0 nM 7.77
CHEMBL278315 GELDANAMYCIN IC50 = 22.0 nM 7.66
CHEMBL560895 SNX-2112 IC50 = 25.0 nM 7.60
CHEMBL2419346 CUDC-305 IC50 = 38.0 nM 7.42
CHEMBL200102 PU-H71 IC50 = 42.0 nM 7.38
CHEMBL109480 TANESPIMYCIN IC50 = 45.0 nM 7.35
CHEMBL14830 ADENOSINE DIPHOSPHATE IC50 = 42159.0 nM 4.38
CHEMBL14249 ADENOSINE TRIPHOSPHATE IC50 = 893677.0 nM -