Assay Detail
Binding
CHEMBL3052328
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3H]IMI from nicotinic acetylcholine receptor in Musca domestica (house fly) head membrane after 90 min by filter binding assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Musca domestica |
| Tissue | Head |
| Subcellular | Membrane |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Nicotinic acetylcholine receptor alpha 5 subunit (CHEMBL2366408) ↗| Type | SINGLE PROTEIN |
| Organism | Musca domestica |
Publication
Insect nicotinic acetylcholine receptor: conserved neonicotinoid specificity of [(3)H]imidacloprid binding site.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 8.18 | 8.74 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL259727 | CLOTHIANIDINE | — | 1 | 8.74 |
| CHEMBL406819 | IMIDACLOPRID | — | 1 | 8.59 |
| CHEMBL148390 | — | — | 1 | 8.15 |
| CHEMBL2251730 | IMIDACLOTHIZ | — | 1 | 7.92 |
| CHEMBL2269823 | — | — | 1 | 7.85 |
| CHEMBL2269822 | DESMETHYLTHIAMETHOXAM | — | 1 | 7.80 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL259727 | CLOTHIANIDINE | IC50 | = | 1.8 | nM | 8.74 |
| CHEMBL406819 | IMIDACLOPRID | IC50 | = | 2.6 | nM | 8.59 |
| CHEMBL148390 | — | IC50 | = | 7.1 | nM | 8.15 |
| CHEMBL2251730 | IMIDACLOTHIZ | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL2269823 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL2269822 | DESMETHYLTHIAMETHOXAM | IC50 | = | 16.0 | nM | 7.80 |