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Assay Detail

CHEMBL3281632

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 1 x 10'-8 M of [1,2,3-3H]-triamcinolone acetonide from glucocorticoid receptor in soluble fraction of mouse L929 cells after 20 hrs
4
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type L929
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucocorticoid receptor (CHEMBL3144)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and biological action of two glucocorticoid alkylating agents.
El Masry AH, Braun VC, Nielsen CJ, Pratt WB.
J Med Chem (1977) 20 : 1134 -1139
PubMed 926113 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 2 - -
Kd 2 7.05 7.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL389621 HYDROCORTISONE 4.0 1 7.37
CHEMBL977 HYDROCORTISONE HEMISUCCINATE ANHYDROUS 4.0 1 6.72
CHEMBL3273978 1 -
CHEMBL1641 HYDROCORTISONE PHOSPHORIC ACID 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL389621 HYDROCORTISONE Kd = 43.0 nM 7.37
CHEMBL977 HYDROCORTISONE HEMISUCCINATE ANHYDROUS Kd = 190.0 nM 6.72
CHEMBL3273978 Inhibition - % -
CHEMBL1641 HYDROCORTISONE PHOSPHORIC ACID Inhibition - % -