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Assay Detail

CHEMBL3379281

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HSP90 in human MDA-kb2 cells assessed as reduction in glucocorticoid receptor-dependent luciferase expression after 18 hrs by firefly luciferase reporter gene assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDA-kb2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Heat shock protein HSP 90-beta (CHEMBL4303)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potential C-terminal-domain inhibitors of heat shock protein 90 derived from a C-terminal peptide helix.
Gavenonis J, Jonas NE, Kritzer JA.
Bioorg Med Chem (2014) 22 : 3989 -3993
PubMed 24984936 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.00 6.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3360777 1 6.14
CHEMBL3360774 1 4.98
CHEMBL389471 COUMERMYCIN A1 1 4.82
CHEMBL3360770 1 4.80
CHEMBL3360775 1 4.78
CHEMBL3360767 1 4.73
CHEMBL3360769 1 4.73
CHEMBL36506 NOVOBIOCIN 4.0 1 -
CHEMBL278315 GELDANAMYCIN 2.0 1 -
CHEMBL3360762 1 -
CHEMBL3360771 1 -
CHEMBL3360772 1 -
CHEMBL3360773 1 -
CHEMBL3360776 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3360777 IC50 = 730.0 nM 6.14
CHEMBL3360774 IC50 = 10400.0 nM 4.98
CHEMBL389471 COUMERMYCIN A1 IC50 = 15300.0 nM 4.82
CHEMBL3360770 IC50 = 15800.0 nM 4.80
CHEMBL3360775 IC50 = 16800.0 nM 4.78
CHEMBL3360767 IC50 = 18600.0 nM 4.73
CHEMBL3360769 IC50 = 18800.0 nM 4.73
CHEMBL36506 NOVOBIOCIN IC50 = 231000.0 nM -
CHEMBL278315 GELDANAMYCIN IC50 < 10.0 nM -
CHEMBL3360762 IC50 > 160000.0 nM -
CHEMBL3360771 IC50 > 120000.0 nM -
CHEMBL3360772 IC50 > 67000.0 nM -
CHEMBL3360773 IC50 = 121000.0 nM -
CHEMBL3360776 IC50 > 27000.0 nM -