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Assay Detail

CHEMBL3381103

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Modulation of BCRP1 mediated drug efflux in mitoxantrone-resistant human MES-SA cells assessed as accumulation of hoechst 33342 incubated for 15 mins prior to rhodamine-123 addition measured after 90 mins by fluorescence analysis
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MES-SA
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Thieno[2,3-b]pyridines--a new class of multidrug resistance (MDR) modulators.
Krauze A, Grinberga S, Krasnova L, Adlere I, Sokolova E, Domracheva I, Shestakova I, Andzans Z, Duburs G.
Bioorg Med Chem (2014) 22 : 5860 -5870
PubMed 25311564 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 27 5.82 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3342124 1 6.70
CHEMBL3342113 1 6.40
CHEMBL3342121 1 6.40
CHEMBL3342127 1 6.22
CHEMBL3344542 1 6.16
CHEMBL3342117 1 6.16
CHEMBL3342122 1 6.10
CHEMBL3342123 1 5.89
CHEMBL3342119 1 5.89
CHEMBL3342116 1 5.82
CHEMBL3342125 1 5.60
CHEMBL3344541 1 5.58
CHEMBL3342118 1 5.58
CHEMBL3342128 1 5.52
CHEMBL3342134 1 5.47
CHEMBL3344543 1 5.44
CHEMBL3342110 1 5.39
CHEMBL6966 VERAPAMIL 4.0 1 4.43
CHEMBL3342120 1 -
CHEMBL3342112 1 -
CHEMBL3342111 1 -
CHEMBL3342126 1 -
CHEMBL3342129 1 -
CHEMBL3342130 1 -
CHEMBL3342131 1 -
CHEMBL3342133 1 -
CHEMBL3342135 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3342124 EC50 = 200.0 nM 6.70
CHEMBL3342113 EC50 = 400.0 nM 6.40
CHEMBL3342121 EC50 = 400.0 nM 6.40
CHEMBL3342127 EC50 = 600.0 nM 6.22
CHEMBL3344542 EC50 = 700.0 nM 6.16
CHEMBL3342117 EC50 = 700.0 nM 6.16
CHEMBL3342122 EC50 = 800.0 nM 6.10
CHEMBL3342123 EC50 = 1300.0 nM 5.89
CHEMBL3342119 EC50 = 1300.0 nM 5.89
CHEMBL3342116 EC50 = 1500.0 nM 5.82
CHEMBL3342125 EC50 = 2500.0 nM 5.60
CHEMBL3344541 EC50 = 2600.0 nM 5.58
CHEMBL3342118 EC50 = 2600.0 nM 5.58
CHEMBL3342128 EC50 = 3000.0 nM 5.52
CHEMBL3342134 EC50 = 3400.0 nM 5.47
CHEMBL3344543 EC50 = 3600.0 nM 5.44
CHEMBL3342110 EC50 = 4100.0 nM 5.39
CHEMBL6966 VERAPAMIL EC50 = 37300.0 nM 4.43
CHEMBL3342120 EC50 - -
CHEMBL3342112 EC50 - -
CHEMBL3342111 EC50 - -
CHEMBL3342126 EC50 - -
CHEMBL3342129 EC50 - -
CHEMBL3342130 EC50 - -
CHEMBL3342131 EC50 - -
CHEMBL3342133 EC50 - -
CHEMBL3342135 EC50 - -