Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3411198

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of purified recombinant FASN TE activity (unknown origin) using 4-MUH as substrate preincubated for 30 mins before substrate addition measured after 1 hr by fluorescence assay
11
Total Activities
6
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty acid synthase (CHEMBL4158)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Repositioning proton pump inhibitors as anticancer drugs by targeting the thioesterase domain of human fatty acid synthase.
Fako VE, Wu X, Pflug B, Liu JY, Zhang JT.
J Med Chem (2015) 58 : 778 -784
PubMed 25513712 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.40 4.53
Ki 4 5.34 5.47
Inhibition 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1503 OMEPRAZOLE 4.0 2 5.47
CHEMBL1502 PANTOPRAZOLE 4.0 3 5.39
CHEMBL480 LANSOPRAZOLE 4.0 2 5.28
CHEMBL1219 RABEPRAZOLE 4.0 2 5.23
CHEMBL547 ISOTRETINOIN 4.0 1 -
CHEMBL404849 SULOCTIDIL 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1503 OMEPRAZOLE Ki = 3400.0 nM 5.47
CHEMBL1502 PANTOPRAZOLE Ki = 4100.0 nM 5.39
CHEMBL480 LANSOPRAZOLE Ki = 5300.0 nM 5.28
CHEMBL1219 RABEPRAZOLE Ki = 5900.0 nM 5.23
CHEMBL1503 OMEPRAZOLE IC50 = 29600.0 nM 4.53
CHEMBL1502 PANTOPRAZOLE IC50 = 36200.0 nM 4.44
CHEMBL480 LANSOPRAZOLE IC50 = 46700.0 nM 4.33
CHEMBL1219 RABEPRAZOLE IC50 = 52000.0 nM 4.28
CHEMBL1502 PANTOPRAZOLE Inhibition >= 40.0 % -
CHEMBL547 ISOTRETINOIN Inhibition >= 40.0 % -
CHEMBL404849 SULOCTIDIL Inhibition >= 40.0 % -