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Assay Detail

CHEMBL3748906

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]DAMGO from human MOR expressed in HEK293 cells preincubated for 1 hr followed by radioligand addition measured after 1 hr by liquid scintillation counting analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis, Biological Evaluation, and Utility of Fluorescent Ligands Targeting the μ-Opioid Receptor.
Schembri LS, Stoddart LA, Briddon SJ, Kellam B, Canals M, Graham B, Scammells PJ.
J Med Chem (2015) 58 : 9754 -9767
PubMed 26632862 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 9.08 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL281786 DIPRENORPHINE 2.0 1 9.70
CHEMBL19019 NALTREXONE 4.0 1 9.70
CHEMBL38874 DAMGO 1 8.90
CHEMBL80 NALOXONE 4.0 1 8.90
CHEMBL278789 DERMORPHIN 1 8.80
CHEMBL1618305 NALOXONAZINE 1 8.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL281786 DIPRENORPHINE Ki = 0.1995 nM 9.70
CHEMBL19019 NALTREXONE Ki = 0.1995 nM 9.70
CHEMBL38874 DAMGO Ki = 1.259 nM 8.90
CHEMBL80 NALOXONE Ki = 1.259 nM 8.90
CHEMBL278789 DERMORPHIN Ki = 1.585 nM 8.80
CHEMBL1618305 NALOXONAZINE Ki = 3.162 nM 8.50