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Assay Detail

CHEMBL3815884

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]PIC from Imidazoline-1 receptor in rat PC12 cell membrane incubated for 30 mins by gamma counting method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type PC-12
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nischarin (CHEMBL5221)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

A combined ligand- and structure-based approach for the identification of rilmenidine-derived compounds which synergize the antitumor effects of doxorubicin.
Vucicevic J, Srdic-Rajic T, Pieroni M, Laurila JM, Perovic V, Tassini S, Azzali E, Costantino G, Glisic S, Agbaba D, Scheinin M, Nikolic K, Radi M, Veljkovic N.
Bioorg Med Chem (2016) 24 : 3174 -3183
PubMed 27265687 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 8.33 9.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2058635 1 9.75
CHEMBL360803 PI-CLONIDINE 1 9.10
CHEMBL266155 BENAZOLINE 1 8.89
CHEMBL17860 LOFEXIDINE 4.0 1 8.25
CHEMBL289480 RILMENIDINE 4.0 1 7.90
CHEMBL1162356 1 7.55
CHEMBL134 CLONIDINE 4.0 1 6.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2058635 Ki = 0.1778 nM 9.75
CHEMBL360803 PI-CLONIDINE Ki = 0.7943 nM 9.10
CHEMBL266155 BENAZOLINE Ki = 1.288 nM 8.89
CHEMBL17860 LOFEXIDINE Ki = 5.623 nM 8.25
CHEMBL289480 RILMENIDINE Ki = 12.59 nM 7.90
CHEMBL1162356 Ki = 28.18 nM 7.55
CHEMBL134 CLONIDINE Ki = 125.89 nM 6.90