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Assay Detail

CHEMBL3865666

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged full length human SYK (1 to 635 end residues) expressed in baculovirus expression system preincubated for 10 mins followed by Blk/Lyntide substrate and ATP addition measured after 45 mins TR-FRET assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase SYK (CHEMBL2599)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Carboxamide Spleen Tyrosine Kinase (Syk) Inhibitors: Leveraging Ground State Interactions To Accelerate Optimization.
Ellis JM, Altman MD, Cash B, Haidle AM, Kubiak RL, Maddess ML, Yan Y, Northrup AB.
ACS Med Chem Lett (2016) 7 : 1151 -1155
PubMed 27994755 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.89 10.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3415583 1 10.22
CHEMBL3414584 1 9.30
CHEMBL3415610 1 9.15
CHEMBL3951922 1 8.30
CHEMBL3943181 1 8.15
CHEMBL3961713 1 8.05
CHEMBL3934210 1 7.62
CHEMBL3972739 1 6.28
CHEMBL3925317 1 6.00
CHEMBL3935657 1 5.80
CHEMBL3963092 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3415583 IC50 = 0.06 nM 10.22
CHEMBL3414584 IC50 = 0.5 nM 9.30
CHEMBL3415610 IC50 = 0.7 nM 9.15
CHEMBL3951922 IC50 = 5.0 nM 8.30
CHEMBL3943181 IC50 = 7.0 nM 8.15
CHEMBL3961713 IC50 = 9.0 nM 8.05
CHEMBL3934210 IC50 = 24.0 nM 7.62
CHEMBL3972739 IC50 = 530.0 nM 6.28
CHEMBL3925317 IC50 = 990.0 nM 6.00
CHEMBL3935657 IC50 = 1600.0 nM 5.80
CHEMBL3963092 IC50 > 10000.0 nM -