Assay Detail
Binding
CHEMBL3866458
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human CDK2/cyclinA expressed in Baculovirus infected T.ni cells using Biotin-aminohexyl-Ala-Arg-Arg-Pro-Met-Ser-Pro-Lys-LysLys-Ala-CONH2 as substrate measured after 20 to 30 mins in presence of [gamma-32P]ATP by scintillation counting method
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | T.ni |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Publication
3-Hydrazinoindolin-2-one derivatives: Chemical classification and investigation of their targets as anticancer agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 8.27 | 8.80 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL409892 | GW305178X | — | 1 | 8.80 |
| CHEMBL269827 | GW276655X | — | 1 | 8.64 |
| CHEMBL411426 | — | — | 1 | 8.60 |
| CHEMBL271160 | — | — | 1 | 8.47 |
| CHEMBL410883 | — | — | 1 | 8.35 |
| CHEMBL407709 | — | — | 1 | 8.15 |
| CHEMBL272389 | — | — | 1 | 7.80 |
| CHEMBL272387 | — | — | 1 | 7.37 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL409892 | GW305178X | IC50 | = | 1.6 | nM | 8.80 |
| CHEMBL269827 | GW276655X | IC50 | = | 2.3 | nM | 8.64 |
| CHEMBL411426 | — | IC50 | = | 2.5 | nM | 8.60 |
| CHEMBL271160 | — | IC50 | = | 3.4 | nM | 8.47 |
| CHEMBL410883 | — | IC50 | = | 4.5 | nM | 8.35 |
| CHEMBL407709 | — | IC50 | = | 7.1 | nM | 8.15 |
| CHEMBL272389 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL272387 | — | IC50 | = | 43.0 | nM | 7.37 |