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Assay Detail

CHEMBL3991776

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry.
243
Total Activities
243
Compounds Tested
1
Activity Types
1
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type K-562, COLO 205, SK-N-BE(2) and MV-4-11
Subcellular Cell lysate
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 8 (CHEMBL2276)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The target landscape of clinical kinase drugs.
Klaeger S, Heinzlmeir S and Wilhelm M et al
Science (2017) 358
PubMed 29191878 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 243 6.04 7.77

Assay Parameters

Parameter Type Value Units Text
Direct Binder Direct Binder - yes

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1950289 TANZISERTIB 2.0 1 7.77
CHEMBL3688339 1 6.82
CHEMBL495727 AT-9283 2.0 1 6.80
CHEMBL482967 CYC-116 1.0 1 6.53
CHEMBL3991929 Cerdulatinib Hydrochloride 1 6.45
CHEMBL281948 K-252A 1 6.26
CHEMBL1078178 MOMELOTINIB 4.0 1 6.22
CHEMBL1614713 CC-401 2.0 1 6.20
CHEMBL3545083 RGB-286638 1.0 1 6.14
CHEMBL3301622 GILTERITINIB 4.0 1 6.13
CHEMBL603469 LESTAURTINIB 3.0 1 6.11
CHEMBL3545328 XL-019 1.0 1 6.07
CHEMBL3600873 MK-5108 1.0 1 5.94
CHEMBL1944698 ZOTIRACICLIB 2.0 1 5.84
CHEMBL2029988 CEP-32496 2.0 1 5.83
CHEMBL488436 AZD-5438 1.0 1 5.78
CHEMBL3301612 ENCORAFENIB 4.0 1 5.73
CHEMBL2035187 PACRITINIB 4.0 1 5.71
CHEMBL2103830 FOSTAMATINIB 4.0 1 5.70
CHEMBL1908397 KW-2449 1.0 1 5.69
CHEMBL1287853 FEDRATINIB 4.0 1 5.65
CHEMBL475251 R-406 2.0 1 5.63
CHEMBL3301610 ABEMACICLIB 4.0 1 5.42
CHEMBL1738757 REBASTINIB 2.0 1 4.58
CHEMBL5416410 DASATINIB 4.0 1 -
CHEMBL3989914 GLESATINIB 2.0 1 -
CHEMBL3545085 XL-228 1.0 1 -
CHEMBL2103839 RIDAFOROLIMUS 3.0 1 -
CHEMBL3188267 CAPMATINIB 4.0 1 -
CHEMBL411907 SONOLISIB 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1950289 TANZISERTIB Kd = 17.0 nM 7.77
CHEMBL3688339 Kd = 151.0 nM 6.82
CHEMBL495727 AT-9283 Kd = 157.0 nM 6.80
CHEMBL482967 CYC-116 Kd = 298.0 nM 6.53
CHEMBL3991929 Cerdulatinib Hydrochloride Kd = 356.0 nM 6.45
CHEMBL281948 K-252A Kd = 551.0 nM 6.26
CHEMBL1078178 MOMELOTINIB Kd = 599.0 nM 6.22
CHEMBL1614713 CC-401 Kd = 636.0 nM 6.20
CHEMBL3545083 RGB-286638 Kd = 731.0 nM 6.14
CHEMBL3301622 GILTERITINIB Kd = 736.0 nM 6.13
CHEMBL603469 LESTAURTINIB Kd = 783.0 nM 6.11
CHEMBL3545328 XL-019 Kd = 844.0 nM 6.07
CHEMBL3600873 MK-5108 Kd = 1148.0 nM 5.94
CHEMBL1944698 ZOTIRACICLIB Kd = 1457.0 nM 5.84
CHEMBL2029988 CEP-32496 Kd = 1471.0 nM 5.83
CHEMBL488436 AZD-5438 Kd = 1676.0 nM 5.78
CHEMBL3301612 ENCORAFENIB Kd = 1865.0 nM 5.73
CHEMBL2035187 PACRITINIB Kd = 1957.0 nM 5.71
CHEMBL2103830 FOSTAMATINIB Kd = 2011.0 nM 5.70
CHEMBL1908397 KW-2449 Kd = 2027.0 nM 5.69
CHEMBL1287853 FEDRATINIB Kd = 2223.0 nM 5.65
CHEMBL475251 R-406 Kd = 2332.0 nM 5.63
CHEMBL3301610 ABEMACICLIB Kd = 3756.0 nM 5.42
CHEMBL1738757 REBASTINIB Kd = 26059.0 nM 4.58
CHEMBL1229517 VEMURAFENIB Kd > 30000.0 nM -
CHEMBL3545396 BMS-690514 Kd > 30000.0 nM -
CHEMBL283120 AXL-1717 Kd > 30000.0 nM -
CHEMBL575448 BMS-754807 Kd > 30000.0 nM -
CHEMBL1234354 PF-04691502 Kd > 30000.0 nM -
CHEMBL2323775 MK-8033 Kd > 30000.0 nM -
CHEMBL2408045 SAPITINIB Kd > 30000.0 nM -
CHEMBL3137336 UPROSERTIB Kd > 30000.0 nM -
CHEMBL300138 ENZASTAURIN Kd > 30000.0 nM -
CHEMBL3699142 Kd > 30000.0 nM -
CHEMBL939 GEFITINIB Kd > 30000.0 nM -
CHEMBL558752 RAF-265 Kd > 30000.0 nM -
CHEMBL14762 SELICICLIB Kd > 30000.0 nM -
CHEMBL1236682 REFAMETINIB Kd > 30000.0 nM -
CHEMBL2396661 ALPELISIB Kd > 30000.0 nM -
CHEMBL3991932 PEXMETINIB Kd > 30000.0 nM -
CHEMBL1236962 OMIPALISIB Kd > 30000.0 nM -
CHEMBL1289601 LENVATINIB Kd > 30000.0 nM -
CHEMBL405130 WHI-P131 Kd > 30000.0 nM -
CHEMBL415049 BARASERTIB Kd > 30000.0 nM -
CHEMBL2110732 DACOMITINIB ANHYDROUS Kd > 30000.0 nM -
CHEMBL1233528 VOLASERTIB Kd > 30000.0 nM -
CHEMBL2103882 TIVANTINIB Kd > 30000.0 nM -
CHEMBL3184679 AZD-4547 Kd > 30000.0 nM -
CHEMBL3991934 Kd > 30000.0 nM -
CHEMBL2364611 GALUNISERTIB Kd > 30000.0 nM -