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Assay Detail

CHEMBL4001852

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]5-HT uptake by SERT primary site (S1) (unknown origin) expressed in African green monkey COS1 cells after 10 mins by TopCount scintillation counting method
17
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent serotonin transporter (CHEMBL228)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

X-ray structure based evaluation of analogs of citalopram: Compounds with increased affinity and selectivity compared with R-citalopram for the allosteric site (S2) on hSERT.
Topiol S, Bang-Andersen B, Sanchez C, Plenge P, Loland CJ, Juhl K, Larsen K, Bregnedal P, Bøgesø KP.
Bioorg Med Chem Lett (2017) 27 : 470 -478
PubMed 28041833 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.40 8.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4072807 1 8.80
CHEMBL1508 ESCITALOPRAM 4.0 1 8.68
CHEMBL4093518 1 8.40
CHEMBL549 CITALOPRAM 4.0 1 8.17
CHEMBL4064213 1 8.10
CHEMBL4070493 3 8.00
CHEMBL4085773 1 7.92
CHEMBL4062666 1 7.89
CHEMBL399730 (R)-CITALOPRAM 1 6.77
CHEMBL4061866 3 6.60
CHEMBL4071311 3 6.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4072807 IC50 = 1.6 nM 8.80
CHEMBL1508 ESCITALOPRAM IC50 = 2.1 nM 8.68
CHEMBL4093518 IC50 = 4.0 nM 8.40
CHEMBL549 CITALOPRAM IC50 = 6.7 nM 8.17
CHEMBL4064213 IC50 = 8.0 nM 8.10
CHEMBL4070493 IC50 = 10.0 nM 8.00
CHEMBL4085773 IC50 = 12.0 nM 7.92
CHEMBL4062666 IC50 = 13.0 nM 7.89
CHEMBL4070493 IC50 = 34.0 nM 7.47
CHEMBL4070493 IC50 = 150.0 nM 6.82
CHEMBL399730 (R)-CITALOPRAM IC50 = 170.0 nM 6.77
CHEMBL4061866 IC50 = 250.0 nM 6.60
CHEMBL4061866 IC50 = 280.0 nM 6.55
CHEMBL4071311 IC50 = 300.0 nM 6.52
CHEMBL4071311 IC50 = 340.0 nM 6.47
CHEMBL4071311 IC50 = 350.0 nM 6.46
CHEMBL4061866 IC50 = 740.0 nM 6.13