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Assay Detail

CHEMBL4033943

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of protein translation in Escherichia coli S30 cell extract using circular DNA with pBESTluc after 60 mins
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Escherichia coli
Confidence 1 — Organism
Curated By Autocuration

Target

Escherichia coli (CHEMBL354)
Type ORGANISM
Organism Escherichia coli

Publication

Covalently linked kanamycin - Ciprofloxacin hybrid antibiotics as a tool to fight bacterial resistance.
Shavit M, Pokrovskaya V, Belakhov V, Baasov T.
Bioorg Med Chem (2017) 25 : 2917 -2925
PubMed 28343755 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.58 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1384 KANAMYCIN 4.0 1 7.52
CHEMBL4074141 1 5.90
CHEMBL4083503 1 5.66
CHEMBL4065520 1 5.19
CHEMBL4089761 1 5.09
CHEMBL4081574 1 4.97
CHEMBL4064500 1 4.72
CHEMBL8 CIPROFLOXACIN 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1384 KANAMYCIN IC50 = 30.0 nM 7.52
CHEMBL4074141 IC50 = 1270.0 nM 5.90
CHEMBL4083503 IC50 = 2190.0 nM 5.66
CHEMBL4065520 IC50 = 6460.0 nM 5.19
CHEMBL4089761 IC50 = 8140.0 nM 5.09
CHEMBL4081574 IC50 = 10690.0 nM 4.97
CHEMBL4064500 IC50 = 19180.0 nM 4.72
CHEMBL8 CIPROFLOXACIN IC50 - -