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Assay Detail

CHEMBL4040854

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Positive allosteric modulation of GABAA receptor alpha1beta2gamma2 (unknown origin) expressed in mouse LTK cells assessed as potentiation of GABA-mediated increase in channel current under holding potential at -80 mV preincubated for 30 secs followed by GABA stimulation for 2 secs by automated patch clamp assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-1/beta-2/gamma-2 (CHEMBL2095172)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Neuroactive Steroids. 2. 3α-Hydroxy-3β-methyl-21-(4-cyano-1H-pyrazol-1'-yl)-19-nor-5β-pregnan-20-one (SAGE-217): A Clinical Next Generation Neuroactive Steroid Positive Allosteric Modulator of the (γ-Aminobutyric Acid)A Receptor.
Martinez Botella G, Salituro FG, Harrison BL, Beresis RT, Bai Z, Blanco MJ, Belfort GM, Dai J, Loya CM, Ackley MA, Althaus AL, Grossman SJ, Hoffmann E, Doherty JJ, Robichaud AJ.
J Med Chem (2017) 60 : 7810 -7819
PubMed 28753313 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 21 6.19 6.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3427532 1 6.90
CHEMBL4073780 1 6.65
CHEMBL207538 BREXANOLONE 4.0 1 6.62
CHEMBL4073679 1 6.54
CHEMBL4105630 ZURANOLONE 4.0 1 6.43
CHEMBL3427530 1 6.01
CHEMBL4103751 1 5.98
CHEMBL4091891 1 5.94
CHEMBL4085062 1 5.83
CHEMBL4062424 1 5.79
CHEMBL4080678 1 5.79
CHEMBL4081622 1 5.75
CHEMBL4098519 1 -
CHEMBL4072311 1 -
CHEMBL4104908 1 -
CHEMBL4084134 1 -
CHEMBL4100583 1 -
CHEMBL4099623 1 -
CHEMBL4071062 1 -
CHEMBL4078922 1 -
CHEMBL4103198 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3427532 EC50 = 125.0 nM 6.90
CHEMBL4073780 EC50 = 225.0 nM 6.65
CHEMBL207538 BREXANOLONE EC50 = 237.0 nM 6.62
CHEMBL4073679 EC50 = 291.0 nM 6.54
CHEMBL4105630 ZURANOLONE EC50 = 375.0 nM 6.43
CHEMBL3427530 EC50 = 978.7 nM 6.01
CHEMBL4103751 EC50 = 1053.0 nM 5.98
CHEMBL4091891 EC50 = 1159.0 nM 5.94
CHEMBL4085062 EC50 = 1496.0 nM 5.83
CHEMBL4062424 EC50 = 1639.0 nM 5.79
CHEMBL4080678 EC50 = 1604.0 nM 5.79
CHEMBL4081622 EC50 = 1760.0 nM 5.75
CHEMBL4098519 EC50 - -
CHEMBL4072311 EC50 > 3000.0 nM -
CHEMBL4104908 EC50 > 3000.0 nM -
CHEMBL4084134 EC50 > 3000.0 nM -
CHEMBL4100583 EC50 > 3000.0 nM -
CHEMBL4099623 EC50 - -
CHEMBL4071062 EC50 > 3000.0 nM -
CHEMBL4078922 EC50 > 10000.0 nM -
CHEMBL4103198 EC50 > 3647.0 nM -