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Assay Detail

CHEMBL4133992

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against HIV NL4.3 harboring renilla luciferase infected in human MT2 cells pretreated for 2 hrs followed by viral infection measured after 48 to 96 hrs post infection by luciferase reporter gene assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Human immunodeficiency virus
Confidence 1 — Organism
Curated By Intermediate

Target

Human immunodeficiency virus (CHEMBL613758)
Type ORGANISM
Organism Human immunodeficiency virus

Publication

Environmentally Friendly Procedure Based on Supercritical Fluid Chromatography and Tandem Mass Spectrometry Molecular Networking for the Discovery of Potent Antiviral Compounds from Euphorbia semiperfoliata.
Nothias LF, Boutet-Mercey S, Cachet X, De La Torre E, Laboureur L, Gallard JF, Retailleau P, Brunelle A, Dorrestein PC, Costa J, Bedoya LM, Roussi F, Leyssen P, Alcami J, Paolini J, Litaudon M, Touboul D.
J Nat Prod (2017) 80 : 2620 -2629
PubMed 28925702 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 6.56 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4165007 1 7.89
CHEMBL4170130 1 7.27
CHEMBL170518 PROSTRATIN 1 6.65
CHEMBL4168419 1 5.59
CHEMBL2375779 1 5.38
CHEMBL4174527 1 -
CHEMBL4169508 1 -
CHEMBL279115 PHORBOL MYRISTATE ACETATE 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4165007 EC50 = 13.0 nM 7.89
CHEMBL4170130 EC50 = 54.0 nM 7.27
CHEMBL170518 PROSTRATIN EC50 = 226.0 nM 6.65
CHEMBL4168419 EC50 = 2560.0 nM 5.59
CHEMBL2375779 EC50 = 4190.0 nM 5.38
CHEMBL4174527 EC50 - -
CHEMBL4169508 EC50 - -
CHEMBL279115 PHORBOL MYRISTATE ACETATE EC50 - -