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Assay Detail

CHEMBL4182255

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ADMET
Inhibition of recombinant human BRD2 bromodomain 1 (49 to 170 residues) using biotin labeled peptide substrate preincubated for 15 mins followed by substrate addition measured after 1 hr by TR-FRET assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 2 (CHEMBL1293289)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Exploiting a water network to achieve enthalpy-driven, bromodomain-selective BET inhibitors.
Shadrick WR, Slavish PJ, Chai SC, Waddell B, Connelly M, Low JA, Tallant C, Young BM, Bharatham N, Knapp S, Boyd VA, Morfouace M, Roussel MF, Chen T, Lee RE, Kiplin Guy R, Shelat AA, Potter PM.
Bioorg Med Chem (2018) 26 : 25 -36
PubMed 29170024 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.75 6.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1957266 1 6.43
CHEMBL2177300 1 6.14
CHEMBL4202664 1 5.85
CHEMBL4205876 1 5.75
CHEMBL4209110 1 5.70
CHEMBL4206677 1 5.61
CHEMBL4210405 1 5.28
CHEMBL4207106 1 5.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1957266 IC50 = 370.0 nM 6.43
CHEMBL2177300 IC50 = 720.0 nM 6.14
CHEMBL4202664 IC50 = 1400.0 nM 5.85
CHEMBL4205876 IC50 = 1770.0 nM 5.75
CHEMBL4209110 IC50 = 2020.0 nM 5.70
CHEMBL4206677 IC50 = 2480.0 nM 5.61
CHEMBL4210405 IC50 = 5210.0 nM 5.28
CHEMBL4207106 IC50 = 5800.0 nM 5.24