Assay Detail
Binding
CHEMBL4187347
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Binding affinity to recombinant human full length N-terminal His6-tagged mitochondrial carbonic anhydrase 5A expressed in Escherichia coli BL21 (DE3) assessed as intrinsic dissociation constant in presence of ANS by fluorescent thermal shift assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Carbonic anhydrase 5A, mitochondrial (CHEMBL4789) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Benzimidazole design, synthesis, and docking to build selective carbonic anhydrase VA inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 20 | 8.71 | 10.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4205449 | — | — | 1 | 10.70 |
| CHEMBL4207063 | — | — | 1 | 10.22 |
| CHEMBL4214894 | — | — | 1 | 10.15 |
| CHEMBL4204729 | — | — | 1 | 9.85 |
| CHEMBL4210750 | — | — | 1 | 9.85 |
| CHEMBL4207242 | — | — | 1 | 9.33 |
| CHEMBL4207577 | — | — | 1 | 9.18 |
| CHEMBL4218335 | — | — | 1 | 9.10 |
| CHEMBL4211504 | — | — | 1 | 9.06 |
| CHEMBL1272188 | — | — | 1 | 8.46 |
| CHEMBL4203615 | — | — | 1 | 8.38 |
| CHEMBL4208414 | — | — | 1 | 8.33 |
| CHEMBL406 | INDAPAMIDE | 4.0 | 1 | 8.16 |
| CHEMBL1272243 | — | — | 1 | 8.16 |
| CHEMBL4215863 | — | — | 1 | 7.89 |
| CHEMBL4204892 | — | — | 1 | 7.83 |
| CHEMBL20 | ACETAZOLAMIDE | 4.0 | 1 | 6.48 |
| CHEMBL4216585 | — | — | 1 | 5.68 |
| CHEMBL4205044 | — | — | 1 | - |
| CHEMBL1272187 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4205449 | — | Kd | = | 0.02 | nM | 10.70 |
| CHEMBL4207063 | — | Kd | = | 0.06 | nM | 10.22 |
| CHEMBL4214894 | — | Kd | = | 0.07 | nM | 10.15 |
| CHEMBL4204729 | — | Kd | = | 0.14 | nM | 9.85 |
| CHEMBL4210750 | — | Kd | = | 0.14 | nM | 9.85 |
| CHEMBL4207242 | — | Kd | = | 0.47 | nM | 9.33 |
| CHEMBL4207577 | — | Kd | = | 0.66 | nM | 9.18 |
| CHEMBL4218335 | — | Kd | = | 0.8 | nM | 9.10 |
| CHEMBL4211504 | — | Kd | = | 0.88 | nM | 9.06 |
| CHEMBL1272188 | — | Kd | = | 3.46 | nM | 8.46 |
| CHEMBL4203615 | — | Kd | = | 4.16 | nM | 8.38 |
| CHEMBL4208414 | — | Kd | = | 4.72 | nM | 8.33 |
| CHEMBL406 | INDAPAMIDE | Kd | = | 6.96 | nM | 8.16 |
| CHEMBL1272243 | — | Kd | = | 6.93 | nM | 8.16 |
| CHEMBL4215863 | — | Kd | = | 13.0 | nM | 7.89 |
| CHEMBL4204892 | — | Kd | = | 14.8 | nM | 7.83 |
| CHEMBL20 | ACETAZOLAMIDE | Kd | = | 333.0 | nM | 6.48 |
| CHEMBL4216585 | — | Kd | = | 2079.0 | nM | 5.68 |
| CHEMBL4205044 | — | Kd | = | 0.008 | nM | - |
| CHEMBL1272187 | — | Kd | = | 0.003 | nM | - |