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Assay Detail

CHEMBL4306811

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Agonist activity at human Muscarinic acetylcholine receptor M2 expressed in HEK cells co-expressing Glosensor construct assessed as decrease in isoproterenol-induced cAMP accumulation incubated for 7 mins in presence of isoproterenol by Glosensor cAMP reagent/plate reader based luminescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M2 (CHEMBL211)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Selective M4 Muscarinic Acetylcholine Receptor Agonists with Novel Carbamate Isosteres.
Yang Q, Lachapelle EA, Kablaoui NM, Webb D, Popiolek M, Grimwood S, Kozak R, O'Connor RE, Lazzaro JT, Butler CR, Zhang L.
ACS Med Chem Lett (2019) 10 : 941 -948
PubMed 31223452 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 5.97 6.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4455058 1 6.60
CHEMBL4515088 1 6.38
CHEMBL4461438 1 5.60
CHEMBL4583597 1 5.29
CHEMBL4446791 1 -
CHEMBL4524686 1 -
CHEMBL4543638 1 -
CHEMBL4541697 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4455058 EC50 = 250.0 nM 6.60
CHEMBL4515088 EC50 = 420.0 nM 6.38
CHEMBL4461438 EC50 = 2520.0 nM 5.60
CHEMBL4583597 EC50 = 5160.0 nM 5.29
CHEMBL4446791 EC50 - -
CHEMBL4524686 EC50 > 8830.0 nM -
CHEMBL4543638 EC50 > 10000.0 nM -
CHEMBL4541697 EC50 > 7350.0 nM -