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Assay Detail

CHEMBL4610313

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Agonist activity at human beta1 adrenoreceptor overexpressed in human HEK293 cells assessed as cAMP accumulation incubated for 60 mins by HTRF assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-1 adrenergic receptor (CHEMBL213)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of 8-(2-amino-1-hydroxyethyl)-6-hydroxy-1,4-benzoxazine-3(4H)-one derivatives as potent β<sub>2</sub>-adrenoceptor agonists.
Yi C, Xing G, Wang S, Li X, Liu Y, Li J, Lin B, Woo AY, Zhang Y, Pan L, Cheng M.
Bioorg Med Chem (2020) 28 : 115178 -115178
PubMed 31753798 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 8.05 8.91

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4645927 1 8.91
CHEMBL4635163 1 8.85
CHEMBL1711 ISOPROTERENOL HYDROCHLORIDE 4.0 1 8.81
CHEMBL4642770 1 8.41
CHEMBL4636255 1 8.38
CHEMBL4646881 1 8.27
CHEMBL4641262 1 8.25
CHEMBL4645203 1 8.09
CHEMBL4645694 1 7.91
CHEMBL4638864 1 7.83
CHEMBL2105743 OLODATEROL HYDROCHLORIDE 4.0 1 7.56
CHEMBL4644638 1 7.54
CHEMBL4634131 1 7.44
CHEMBL1263 SALMETEROL 4.0 1 6.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4645927 EC50 = 1.23 nM 8.91
CHEMBL4635163 EC50 = 1.413 nM 8.85
CHEMBL1711 ISOPROTERENOL HYDROCHLORIDE EC50 = 1.549 nM 8.81
CHEMBL4642770 EC50 = 3.89 nM 8.41
CHEMBL4636255 EC50 = 4.169 nM 8.38
CHEMBL4646881 EC50 = 5.37 nM 8.27
CHEMBL4641262 EC50 = 5.623 nM 8.25
CHEMBL4645203 EC50 = 8.128 nM 8.09
CHEMBL4645694 EC50 = 12.3 nM 7.91
CHEMBL4638864 EC50 = 14.79 nM 7.83
CHEMBL2105743 OLODATEROL HYDROCHLORIDE EC50 = 27.54 nM 7.56
CHEMBL4644638 EC50 = 28.84 nM 7.54
CHEMBL4634131 EC50 = 36.31 nM 7.44
CHEMBL1263 SALMETEROL EC50 = 316.23 nM 6.50