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Assay Detail

CHEMBL4666433

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal DYKDDDDK-tagged MNK1(1 to 424 residues) expressed in baculovirus expression system using TATKSGSTTKNR as substrate preincubated for 10 mins followed by ATP addition and measured after 40 mins by luminescence based assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Design, synthesis and biological evaluation of pyridone-aminal derivatives as MNK1/2 inhibitors.
Yuan X,Wu H,Bu H,Zheng P,Zhou J,Zhang H
Bioorg Med Chem (2019) 27 : 1211 -1225
PubMed 30824167 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 7.14 8.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4073443 TOMIVOSERTIB 2.0 1 8.48
CHEMBL4757546 1 8.15
CHEMBL4796087 1 8.06
CHEMBL4780209 1 7.98
CHEMBL4754824 1 7.68
CHEMBL4787265 1 7.29
CHEMBL4740694 1 7.28
CHEMBL4793971 1 7.26
CHEMBL4741081 1 7.16
CHEMBL4797669 1 6.90
CHEMBL4760245 1 6.80
CHEMBL4751127 1 6.62
CHEMBL4784364 1 6.41
CHEMBL4741111 1 6.38
CHEMBL4757777 1 6.33
CHEMBL4743829 1 6.32
CHEMBL4752750 1 6.32
CHEMBL4747129 1 -
CHEMBL4791921 1 -
CHEMBL4791161 1 -
CHEMBL4776154 1 -
CHEMBL4746641 1 -
CHEMBL4792146 1 -
CHEMBL4797682 1 -
CHEMBL4752964 1 -
CHEMBL4747093 1 -
CHEMBL4747663 1 -
CHEMBL4757325 1 -
CHEMBL4754789 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4073443 TOMIVOSERTIB IC50 = 3.3 nM 8.48
CHEMBL4757546 IC50 = 7.0 nM 8.15
CHEMBL4796087 IC50 = 8.7 nM 8.06
CHEMBL4780209 IC50 = 10.5 nM 7.98
CHEMBL4754824 IC50 = 20.7 nM 7.68
CHEMBL4787265 IC50 = 51.2 nM 7.29
CHEMBL4740694 IC50 = 52.3 nM 7.28
CHEMBL4793971 IC50 = 55.2 nM 7.26
CHEMBL4741081 IC50 = 68.9 nM 7.16
CHEMBL4797669 IC50 = 125.0 nM 6.90
CHEMBL4760245 IC50 = 159.8 nM 6.80
CHEMBL4751127 IC50 = 239.8 nM 6.62
CHEMBL4784364 IC50 = 390.0 nM 6.41
CHEMBL4741111 IC50 = 422.0 nM 6.38
CHEMBL4757777 IC50 = 466.0 nM 6.33
CHEMBL4743829 IC50 = 479.0 nM 6.32
CHEMBL4752750 IC50 = 476.0 nM 6.32
CHEMBL4747129 IC50 > 500.0 nM -
CHEMBL4791921 IC50 > 500.0 nM -
CHEMBL4791161 IC50 > 500.0 nM -
CHEMBL4776154 IC50 > 500.0 nM -
CHEMBL4746641 IC50 > 500.0 nM -
CHEMBL4792146 IC50 > 500.0 nM -
CHEMBL4797682 IC50 > 500.0 nM -
CHEMBL4752964 IC50 > 500.0 nM -
CHEMBL4747093 IC50 > 500.0 nM -
CHEMBL4747663 IC50 > 500.0 nM -
CHEMBL4757325 IC50 > 500.0 nM -
CHEMBL4754789 IC50 > 500.0 nM -