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Assay Detail

CHEMBL4714828

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human His-tagged FGFR4 (781 to 1338 residues) cytoplasmic domain expressed in baculovirus expression system using Tyr 04 peptide as substrate incubated for 1 hr in presence of ATP by Z'-LYTE assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fibroblast growth factor receptor 4 (CHEMBL3973)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Investigation of Covalent Warheads in the Design of 2-Aminopyrimidine-based FGFR4 Inhibitors.
Deng W,Chen X,Jiang K,Song X,Huang M,Tu ZC,Zhang Z,Lin X,Ortega R,Patterson AV,Smaill JB,Ding K,Chen S,Chen Y,Lu X
ACS Med Chem Lett (2021) 12 : 647 -652
PubMed 33859803 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.99 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3908979 FGF401 2.0 1 8.00
CHEMBL4742889 1 7.80
CHEMBL4757810 1 7.35
CHEMBL4785949 1 7.28
CHEMBL4061083 1 7.14
CHEMBL4740775 1 6.17
CHEMBL4763652 1 5.21
CHEMBL4750816 1 -
CHEMBL4748301 1 -
CHEMBL4739914 1 -
CHEMBL4799486 1 -
CHEMBL4796512 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3908979 FGF401 IC50 = 10.0 nM 8.00
CHEMBL4742889 IC50 = 16.0 nM 7.80
CHEMBL4757810 IC50 = 45.0 nM 7.35
CHEMBL4785949 IC50 = 53.0 nM 7.28
CHEMBL4061083 IC50 = 72.0 nM 7.14
CHEMBL4740775 IC50 = 670.0 nM 6.17
CHEMBL4763652 IC50 = 6100.0 nM 5.21
CHEMBL4750816 IC50 > 10000.0 nM -
CHEMBL4748301 IC50 > 10000.0 nM -
CHEMBL4739914 IC50 > 10000.0 nM -
CHEMBL4799486 IC50 > 10000.0 nM -
CHEMBL4796512 IC50 > 10000.0 nM -