Assay Detail
Binding
CHEMBL4880040
Review assay metadata, readout intent, target linkage, and publication context from the same page.
GPCRScan assay: inhibition of NET
203
Total Activities
181
Compounds Tested
2
Activity Types
12
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Sodium-dependent noradrenaline transporter (CHEMBL222) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
SGC PDSP GPCRScan assays
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 181 | - | - |
| Ki | 22 | 5.54 | 6.09 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Parental cells | Parental cells | - | — | stable HEK |
| PMID | PMID | - | — | 2086242/17156375 |
| Protein length | Protein length | - | — | full-length |
| Radioligand conc. used [nM] | Radioligand conc. used [nM] | - | — | 1.3 – 5.0 |
| Radioligand Kd | Radioligand Kd | 3.83 | nM | — |
| Radioligand name | Radioligand name | - | — | [3H]-Nisoxetine |
| Radioligand pKd | Radioligand pKd | 8.42 | — | — |
| Radioligand pKd ± SEM | Radioligand pKd ± SEM | 0.06 | — | — |
| Reference ligand | Reference ligand | - | — | Desipramine |
| Reference ligand Ki | Reference ligand Ki | 2.24 | nM | — |
| Reference ligand pKi | Reference ligand pKi | 8.65 | — | — |
| Reference ligand pKi ± SEM | Reference ligand pKi ± SEM | 0.01 | — | — |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3609749 | — | — | 3 | 6.09 |
| CHEMBL4535474 | BAY-293 | — | 3 | 6.05 |
| CHEMBL4778870 | TP-040 | — | 3 | 5.72 |
| CHEMBL4520788 | BAY-294 | — | 3 | 5.68 |
| CHEMBL4558518 | GSI-NC | — | 3 | 5.62 |
| CHEMBL5082184 | — | — | 3 | 5.56 |
| CHEMBL4748908 | TP-040n | — | 3 | 5.52 |
| CHEMBL3685796 | MRL-SYKi | — | 3 | 5.11 |
| CHEMBL5306285 | — | — | 3 | 5.08 |
| CHEMBL4526351 | BAY-309 | — | 3 | 5.00 |
| CHEMBL4522773 | BAY-827 | — | 1 | - |
| CHEMBL4585243 | BAY-7598 | — | 1 | - |
| CHEMBL4539943 | BAY-694 | — | 1 | - |
| CHEMBL4521594 | BAY-1797 | — | 1 | - |
| CHEMBL4516843 | BAY-207 | — | 1 | - |
| CHEMBL4537788 | BAY-784 | — | 1 | - |
| CHEMBL4564312 | BAY-786 | — | 1 | - |
| CHEMBL4448899 | BAY-876 | — | 1 | - |
| CHEMBL4552180 | BAY-588 | — | 1 | - |
| CHEMBL4445670 | BAY-885 | — | 1 | - |
| CHEMBL4593677 | BAY-474 | — | 1 | - |
| CHEMBL4066040 | A-1596584 | — | 1 | - |
| CHEMBL4093612 | A-1596586 | — | 1 | - |
| CHEMBL332794 | A-192621 | — | 1 | - |
| CHEMBL4513786 | A-545 | — | 1 | - |
| CHEMBL111612 | ABT-546 | — | 1 | - |
| CHEMBL4526341 | A-108 | — | 1 | - |
| CHEMBL4586794 | ABT-100 | — | 1 | - |
| CHEMBL3701197 | A-226 | — | 1 | - |
| CHEMBL3697701 | A-079 | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3609749 | — | Ki | = | 812.83 | nM | 6.09 |
| CHEMBL3609749 | — | Ki | = | 808.0 | nM | 6.09 |
| CHEMBL4535474 | BAY-293 | Ki | = | 881.05 | nM | 6.05 |
| CHEMBL4535474 | BAY-293 | Ki | = | 982.16 | nM | 6.01 |
| CHEMBL4778870 | TP-040 | Ki | = | 1905.46 | nM | 5.72 |
| CHEMBL4778870 | TP-040 | Ki | = | 1935.54 | nM | 5.71 |
| CHEMBL4520788 | BAY-294 | Ki | = | 2089.3 | nM | 5.68 |
| CHEMBL4520788 | BAY-294 | Ki | = | 2083.21 | nM | 5.68 |
| CHEMBL4558518 | GSI-NC | Ki | = | 2427.0 | nM | 5.62 |
| CHEMBL4558518 | GSI-NC | Ki | = | 2511.89 | nM | 5.60 |
| CHEMBL5082184 | — | Ki | = | 2754.23 | nM | 5.56 |
| CHEMBL5082184 | — | Ki | = | 2744.73 | nM | 5.56 |
| CHEMBL4748908 | TP-040n | Ki | = | 3019.95 | nM | 5.52 |
| CHEMBL4748908 | TP-040n | Ki | = | 3112.25 | nM | 5.51 |
| CHEMBL3685796 | MRL-SYKi | Ki | = | 7713.0 | nM | 5.11 |
| CHEMBL3685796 | MRL-SYKi | Ki | = | 7943.28 | nM | 5.10 |
| CHEMBL5306285 | — | Ki | = | 8271.8 | nM | 5.08 |
| CHEMBL5306285 | — | Ki | = | 8317.64 | nM | 5.08 |
| CHEMBL4526351 | BAY-309 | Ki | = | 10000.0 | nM | 5.00 |
| CHEMBL4526351 | BAY-309 | Ki | = | 10000.0 | nM | 5.00 |
| CHEMBL4798829 | — | Inhibition | = | -23.56 | % | - |
| CHEMBL4075278 | — | Inhibition | = | 0.76 | % | - |
| CHEMBL4514203 | BAY-390 | Inhibition | = | 0.78 | % | - |
| CHEMBL4537673 | BAY-985 | Inhibition | = | -20.01 | % | - |
| CHEMBL4538591 | BAY-440 | Inhibition | = | -29.45 | % | - |
| CHEMBL4580220 | BAY-3827 | Inhibition | = | 2.8 | % | - |
| CHEMBL4630642 | BAY-974 | Inhibition | = | 2.85 | % | - |
| CHEMBL4456585 | WM-1119 | Inhibition | = | -15.5 | % | - |
| CHEMBL4520293 | (R)-ZINC-3573 | Inhibition | = | 45.35 | % | - |
| CHEMBL4534980 | (S)-ZINC-3573 | Inhibition | = | 2.02 | % | - |
| CHEMBL4460070 | ZINC32547799 | Inhibition | = | 43.56 | % | - |
| CHEMBL4088216 | TP-030-1 | Inhibition | = | -12.17 | % | - |
| CHEMBL4549667 | TP-030-2 | Inhibition | = | -7.47 | % | - |
| CHEMBL4551635 | BAY-069 | Inhibition | = | -1.01 | % | - |
| CHEMBL4643852 | BAY-6672 | Inhibition | = | -12.93 | % | - |
| CHEMBL4545807 | BAY-070 | Inhibition | = | -3.53 | % | - |
| CHEMBL4639128 | GSK778 | Inhibition | = | 34.5 | % | - |
| CHEMBL4637208 | GSK973 | Inhibition | = | 0.32 | % | - |
| CHEMBL4638939 | GSK943 | Inhibition | = | -37.31 | % | - |
| CHEMBL1800285 | MSD-M1PAM | Inhibition | = | 24.71 | % | - |
| CHEMBL2204283 | MSD-M1PAM-NC | Inhibition | = | 40.55 | % | - |
| CHEMBL3286797 | PF-04418948 | Inhibition | = | 11.01 | % | - |
| CHEMBL4552554 | PF-04475866 | Inhibition | = | 16.75 | % | - |
| CHEMBL4530740 | BI 1950 | Inhibition | = | -2.81 | % | - |
| CHEMBL4577237 | BI 9446 | Inhibition | = | -16.93 | % | - |
| CHEMBL3137331 | DEFACTINIB | Inhibition | = | 7.81 | % | - |
| CHEMBL4518706 | PF-00911705 | Inhibition | = | 3.15 | % | - |
| CHEMBL4568735 | NVS-MALT1 | Inhibition | = | -28.35 | % | - |
| CHEMBL4584981 | NVS-MALT1-C | Inhibition | = | -20.65 | % | - |
| CHEMBL4282264 | A-485 | Inhibition | = | -3.76 | % | - |