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Assay Detail

CHEMBL5107058

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-NDP-alpha-MSH from human MC4R expressed in HEK2936E cell membrane measured after 16 to 23 hrs by 1450 microbeta trilux scintillation proximity assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK2936E
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 4 (CHEMBL259)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Late-Stage Functionalization with Cysteine Staples Generates Potent and Selective Melanocortin Receptor-1 Agonists.
White AM, Dellsén A, Larsson N, Kaas Q, Jansen F, Plowright AT, Knerr L, Durek T, Craik DJ.
J Med Chem (2022) 65 : 12956 -12969
PubMed 36167503 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 31 7.11 9.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5175981 1 9.04
CHEMBL430239 MELATONAN 1 8.90
CHEMBL3301624 SETMELANOTIDE 4.0 1 8.37
CHEMBL5185945 1 7.70
CHEMBL2070241 BREMELANOTIDE 4.0 1 7.70
CHEMBL5203986 1 7.65
CHEMBL5181812 1 7.51
CHEMBL5185775 1 7.51
CHEMBL5176443 1 7.48
CHEMBL5192329 1 7.48
CHEMBL5191309 1 7.47
CHEMBL5175487 1 7.45
CHEMBL5190042 1 7.36
CHEMBL5170533 1 7.35
CHEMBL5178164 1 7.34
CHEMBL5172738 1 7.21
CHEMBL5195641 1 7.09
CHEMBL5185405 1 7.09
CHEMBL5204285 1 7.04
CHEMBL5207936 1 6.78
CHEMBL5179373 1 6.77
CHEMBL5179081 1 6.58
CHEMBL5182345 1 6.53
CHEMBL5187932 1 6.52
CHEMBL5199932 1 6.46
CHEMBL5196407 1 6.34
CHEMBL5177494 1 6.12
CHEMBL5181752 1 6.05
CHEMBL5195937 1 5.93
CHEMBL5175444 1 5.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5175981 Ki = 0.912 nM 9.04
CHEMBL430239 MELATONAN Ki = 1.259 nM 8.90
CHEMBL3301624 SETMELANOTIDE Ki = 4.266 nM 8.37
CHEMBL5185945 Ki = 19.95 nM 7.70
CHEMBL2070241 BREMELANOTIDE Ki = 19.95 nM 7.70
CHEMBL5203986 Ki = 22.39 nM 7.65
CHEMBL5181812 Ki = 30.9 nM 7.51
CHEMBL5185775 Ki = 30.9 nM 7.51
CHEMBL5176443 Ki = 33.11 nM 7.48
CHEMBL5192329 Ki = 33.11 nM 7.48
CHEMBL5191309 Ki = 33.88 nM 7.47
CHEMBL5175487 Ki = 35.48 nM 7.45
CHEMBL5190042 Ki = 43.65 nM 7.36
CHEMBL5170533 Ki = 44.67 nM 7.35
CHEMBL5178164 Ki = 45.71 nM 7.34
CHEMBL5172738 Ki = 61.66 nM 7.21
CHEMBL5195641 Ki = 81.28 nM 7.09
CHEMBL5185405 Ki = 81.28 nM 7.09
CHEMBL5204285 Ki = 91.2 nM 7.04
CHEMBL5207936 Ki = 165.96 nM 6.78
CHEMBL5179373 Ki = 169.82 nM 6.77
CHEMBL5179081 Ki = 263.03 nM 6.58
CHEMBL5182345 Ki = 295.12 nM 6.53
CHEMBL5187932 Ki = 302.0 nM 6.52
CHEMBL5199932 Ki = 346.74 nM 6.46
CHEMBL5196407 Ki = 457.09 nM 6.34
CHEMBL5177494 Ki = 758.58 nM 6.12
CHEMBL5181752 Ki = 891.25 nM 6.05
CHEMBL5195937 Ki = 1174.9 nM 5.93
CHEMBL5175444 Ki = 1288.25 nM 5.89
CHEMBL5208830 Ki = 2137.96 nM 5.67