Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5133049

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity against human GPBAR1 expressed in HEK293T cells assessed as transactivation of cAMP-responsive element incubated for 18 hrs by Dual-Luciferase reporter assay based luminometer analysis
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled bile acid receptor 1 (CHEMBL5409)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural Basis for Developing Multitarget Compounds Acting on Cysteinyl Leukotriene Receptor 1 and G-Protein-Coupled Bile Acid Receptor 1.
Fiorillo B, Sepe V, Conflitti P, Roselli R, Biagioli M, Marchianò S, De Luca P, Baronissi G, Rapacciuolo P, Cassiano C, Catalanotti B, Zampella A, Limongelli V, Fiorucci S.
J Med Chem (2021) 64 : 16512 -16529
PubMed 34767347 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 5.65 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5192002 1 7.00
CHEMBL5173976 1 6.77
CHEMBL18775 1 6.30
CHEMBL5189181 1 6.00
CHEMBL5172427 1 5.75
CHEMBL8747 REV-5901 1 5.60
CHEMBL5169385 1 5.52
CHEMBL5174405 1 5.13
CHEMBL5202995 1 4.78
CHEMBL5177797 1 4.70
CHEMBL5185938 1 4.64
CHEMBL5205902 1 -
CHEMBL5181326 1 -
CHEMBL5173307 1 -
CHEMBL5193617 1 -
CHEMBL5189686 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5192002 EC50 = 100.0 nM 7.00
CHEMBL5173976 EC50 = 170.0 nM 6.77
CHEMBL18775 EC50 = 500.0 nM 6.30
CHEMBL5189181 EC50 = 1000.0 nM 6.00
CHEMBL5172427 EC50 = 1800.0 nM 5.75
CHEMBL8747 REV-5901 EC50 = 2500.0 nM 5.60
CHEMBL5169385 EC50 = 3000.0 nM 5.52
CHEMBL5174405 EC50 = 7400.0 nM 5.13
CHEMBL5202995 EC50 = 16500.0 nM 4.78
CHEMBL5177797 EC50 = 20000.0 nM 4.70
CHEMBL5185938 EC50 = 23000.0 nM 4.64
CHEMBL5205902 EC50 - -
CHEMBL5181326 EC50 - -
CHEMBL5173307 EC50 - -
CHEMBL5193617 EC50 - -
CHEMBL5189686 EC50 - -