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Assay Detail

CHEMBL5369666

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at Gal4-fused human full length Nurr1 transfected in human HEK293T cells co-transfected with pFR-Luc/pRL-SV40 assessed as activation of luciferase activity incubated for 16 hrs by Dual-Glo luciferase assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nuclear receptor subfamily 4 group A member 2 (CHEMBL5002)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Guided Design of Nurr1 Agonists Derived from the Natural Ligand Dihydroxyindole.
Sai M, Vietor J, Kornmayer M, Egner M, López-García Ú, Höfner G, Pabel J, Marschner JA, Wein T, Merk D.
J Med Chem (2023) 66 : 13556 -13567
PubMed 37751901 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 5.21 6.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1201753 PITAVASTATIN 4.0 1 6.92
CHEMBL2220442 FLUVASTATIN 4.0 1 5.72
CHEMBL5415710 1 5.52
CHEMBL5405956 1 5.52
CHEMBL5399464 1 5.30
CHEMBL5431873 1 5.22
CHEMBL5438087 1 4.92
CHEMBL5416432 1 4.80
CHEMBL5406325 1 4.55
CHEMBL682 AMODIAQUINE 4.0 1 4.44
CHEMBL555013 1 4.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1201753 PITAVASTATIN EC50 = 120.0 nM 6.92
CHEMBL2220442 FLUVASTATIN EC50 = 1900.0 nM 5.72
CHEMBL5415710 EC50 = 3000.0 nM 5.52
CHEMBL5405956 EC50 = 3000.0 nM 5.52
CHEMBL5399464 EC50 = 5000.0 nM 5.30
CHEMBL5431873 EC50 = 6000.0 nM 5.22
CHEMBL5438087 EC50 = 12000.0 nM 4.92
CHEMBL5416432 EC50 = 16000.0 nM 4.80
CHEMBL5406325 EC50 = 28000.0 nM 4.55
CHEMBL682 AMODIAQUINE EC50 = 36000.0 nM 4.44
CHEMBL555013 EC50 = 40000.0 nM 4.40