Assay Detail
Binding
CHEMBL5442181
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PDSP Secondary Binding target: HTR5A - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 30 | 6.18 | 8.06 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1256863 | — | — | 1 | 8.06 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | 4.0 | 1 | 7.50 |
| CHEMBL1256844 | — | — | 1 | 7.37 |
| CHEMBL2164327 | — | — | 1 | 7.12 |
| CHEMBL267777 | RITANSERIN | 2.0 | 1 | 6.99 |
| CHEMBL589390 | LATREPIRDINE | 3.0 | 1 | 6.97 |
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 6.75 |
| CHEMBL1516474 | TEGASEROD MALEATE | 4.0 | 1 | 6.70 |
| CHEMBL405355 | NIGULDIPINE | 2.0 | 1 | 6.37 |
| CHEMBL214957 | — | — | 1 | 6.25 |
| CHEMBL540612 | — | — | 1 | 6.12 |
| CHEMBL1558 | PRAZOSIN HYDROCHLORIDE | 4.0 | 1 | 6.10 |
| CHEMBL1766103 | — | — | 1 | 6.07 |
| CHEMBL1200776 | CINACALCET HYDROCHLORIDE | 4.0 | 1 | 6.01 |
| CHEMBL1836317 | — | — | 1 | 5.98 |
| CHEMBL2107572 | TECALCET HYDROCHLORIDE | 2.0 | 1 | 5.96 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | 4.0 | 1 | 5.96 |
| CHEMBL5089005 | — | — | 1 | 5.93 |
| CHEMBL103769 | — | — | 1 | 5.81 |
| CHEMBL542139 | — | — | 1 | 5.72 |
| CHEMBL3342358 | — | — | 1 | 5.68 |
| CHEMBL4743495 | — | — | 1 | 5.62 |
| CHEMBL531742 | — | — | 1 | 5.61 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | 4.0 | 1 | 5.48 |
| CHEMBL300555 | SPIROXATRINE | 2.0 | 1 | 5.42 |
| CHEMBL83894 | FANANSERIN | 2.0 | 1 | 5.17 |
| CHEMBL500 | PINDOLOL | 4.0 | 1 | 5.15 |
| CHEMBL23327 | PURVALANOLA | — | 1 | 5.12 |
| CHEMBL2057618 | — | — | 1 | - |
| CHEMBL1334465 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1256863 | — | Ki | = | 8.639 | nM | 8.06 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | Ki | = | 31.3 | nM | 7.50 |
| CHEMBL1256844 | — | Ki | = | 42.29 | nM | 7.37 |
| CHEMBL2164327 | — | Ki | = | 75.19 | nM | 7.12 |
| CHEMBL267777 | RITANSERIN | Ki | = | 101.52 | nM | 6.99 |
| CHEMBL589390 | LATREPIRDINE | Ki | = | 107.43 | nM | 6.97 |
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 177.79 | nM | 6.75 |
| CHEMBL1516474 | TEGASEROD MALEATE | Ki | = | 200.91 | nM | 6.70 |
| CHEMBL405355 | NIGULDIPINE | Ki | = | 430.53 | nM | 6.37 |
| CHEMBL214957 | — | Ki | = | 557.12 | nM | 6.25 |
| CHEMBL540612 | — | Ki | = | 754.39 | nM | 6.12 |
| CHEMBL1558 | PRAZOSIN HYDROCHLORIDE | Ki | = | 799.65 | nM | 6.10 |
| CHEMBL1766103 | — | Ki | = | 847.23 | nM | 6.07 |
| CHEMBL1200776 | CINACALCET HYDROCHLORIDE | Ki | = | 971.52 | nM | 6.01 |
| CHEMBL1836317 | — | Ki | = | 1037.41 | nM | 5.98 |
| CHEMBL2107572 | TECALCET HYDROCHLORIDE | Ki | = | 1091.06 | nM | 5.96 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | Ki | = | 1092.07 | nM | 5.96 |
| CHEMBL5089005 | — | Ki | = | 1161.63 | nM | 5.93 |
| CHEMBL103769 | — | Ki | = | 1548.1 | nM | 5.81 |
| CHEMBL542139 | — | Ki | = | 1909.64 | nM | 5.72 |
| CHEMBL3342358 | — | Ki | = | 2109.85 | nM | 5.68 |
| CHEMBL4743495 | — | Ki | = | 2398.47 | nM | 5.62 |
| CHEMBL531742 | — | Ki | = | 2452.73 | nM | 5.61 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | Ki | = | 3293.06 | nM | 5.48 |
| CHEMBL300555 | SPIROXATRINE | Ki | = | 3797.96 | nM | 5.42 |
| CHEMBL83894 | FANANSERIN | Ki | = | 6725.89 | nM | 5.17 |
| CHEMBL500 | PINDOLOL | Ki | = | 7048.55 | nM | 5.15 |
| CHEMBL23327 | PURVALANOLA | Ki | = | 7540.5 | nM | 5.12 |
| CHEMBL2057618 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL1334465 | — | Ki | > | 10000.0 | nM | - |