Assay Detail
Binding
CHEMBL5442193
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PDSP Secondary Binding target: ADRA1B - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
37
Total Activities
37
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 37 | 5.97 | 8.69 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1558 | PRAZOSIN HYDROCHLORIDE | 4.0 | 1 | 8.69 |
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 7.24 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | 4.0 | 1 | 6.92 |
| CHEMBL24778 | SILODOSIN | 4.0 | 1 | 6.71 |
| CHEMBL543113 | — | — | 1 | 6.58 |
| CHEMBL540612 | — | — | 1 | 6.57 |
| CHEMBL79834 | SPIRAMIDE | 2.0 | 1 | 6.53 |
| CHEMBL300555 | SPIROXATRINE | 2.0 | 1 | 6.50 |
| CHEMBL405355 | NIGULDIPINE | 2.0 | 1 | 6.43 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | 4.0 | 1 | 6.24 |
| CHEMBL267777 | RITANSERIN | 2.0 | 1 | 6.22 |
| CHEMBL83894 | FANANSERIN | 2.0 | 1 | 6.07 |
| CHEMBL589390 | LATREPIRDINE | 3.0 | 1 | 5.88 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | 4.0 | 1 | 5.71 |
| CHEMBL103769 | — | — | 1 | 5.69 |
| CHEMBL542139 | — | — | 1 | 5.58 |
| CHEMBL1705 | CLONIDINE HYDROCHLORIDE | 4.0 | 1 | 5.58 |
| CHEMBL1516474 | TEGASEROD MALEATE | 4.0 | 1 | 5.58 |
| CHEMBL5076314 | — | — | 1 | 5.57 |
| CHEMBL1200776 | CINACALCET HYDROCHLORIDE | 4.0 | 1 | 5.54 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | 4.0 | 1 | 5.54 |
| CHEMBL2106195 | DEXMEDETOMIDINE HYDROCHLORIDE | 4.0 | 1 | 5.53 |
| CHEMBL1257131 | RAUWOLSCINE HYDROCHLORIDE | — | 1 | 5.50 |
| CHEMBL531742 | — | — | 1 | 5.45 |
| CHEMBL1200809 | AZELASTINE HYDROCHLORIDE | 4.0 | 1 | 5.44 |
| CHEMBL1707 | LOPERAMIDE HYDROCHLORIDE | 4.0 | 1 | 5.43 |
| CHEMBL74355 | VOLINANSERIN | 3.0 | 1 | 5.42 |
| CHEMBL2062257 | — | — | 1 | 5.38 |
| CHEMBL1256876 | — | — | 1 | 5.38 |
| CHEMBL1095777 | INDACATEROL | 4.0 | 1 | 5.36 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1558 | PRAZOSIN HYDROCHLORIDE | Ki | = | 2.033 | nM | 8.69 |
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 57.94 | nM | 7.24 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | Ki | = | 118.74 | nM | 6.92 |
| CHEMBL24778 | SILODOSIN | Ki | = | 195.79 | nM | 6.71 |
| CHEMBL543113 | — | Ki | = | 263.45 | nM | 6.58 |
| CHEMBL540612 | — | Ki | = | 268.84 | nM | 6.57 |
| CHEMBL79834 | SPIRAMIDE | Ki | = | 295.63 | nM | 6.53 |
| CHEMBL300555 | SPIROXATRINE | Ki | = | 319.41 | nM | 6.50 |
| CHEMBL405355 | NIGULDIPINE | Ki | = | 368.13 | nM | 6.43 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | Ki | = | 572.4 | nM | 6.24 |
| CHEMBL267777 | RITANSERIN | Ki | = | 598.27 | nM | 6.22 |
| CHEMBL83894 | FANANSERIN | Ki | = | 847.42 | nM | 6.07 |
| CHEMBL589390 | LATREPIRDINE | Ki | = | 1305.27 | nM | 5.88 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | Ki | = | 1969.7 | nM | 5.71 |
| CHEMBL103769 | — | Ki | = | 2030.25 | nM | 5.69 |
| CHEMBL542139 | — | Ki | = | 2661.64 | nM | 5.58 |
| CHEMBL1516474 | TEGASEROD MALEATE | Ki | = | 2613.36 | nM | 5.58 |
| CHEMBL1705 | CLONIDINE HYDROCHLORIDE | Ki | = | 2627.54 | nM | 5.58 |
| CHEMBL5076314 | — | Ki | = | 2715.19 | nM | 5.57 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | Ki | = | 2902.69 | nM | 5.54 |
| CHEMBL1200776 | CINACALCET HYDROCHLORIDE | Ki | = | 2889.68 | nM | 5.54 |
| CHEMBL2106195 | DEXMEDETOMIDINE HYDROCHLORIDE | Ki | = | 2977.83 | nM | 5.53 |
| CHEMBL1257131 | RAUWOLSCINE HYDROCHLORIDE | Ki | = | 3136.53 | nM | 5.50 |
| CHEMBL531742 | — | Ki | = | 3537.53 | nM | 5.45 |
| CHEMBL1200809 | AZELASTINE HYDROCHLORIDE | Ki | = | 3638.31 | nM | 5.44 |
| CHEMBL1707 | LOPERAMIDE HYDROCHLORIDE | Ki | = | 3686.38 | nM | 5.43 |
| CHEMBL74355 | VOLINANSERIN | Ki | = | 3807.59 | nM | 5.42 |
| CHEMBL1256876 | — | Ki | = | 4207.75 | nM | 5.38 |
| CHEMBL2062257 | — | Ki | = | 4196.63 | nM | 5.38 |
| CHEMBL1095777 | INDACATEROL | Ki | = | 4322.65 | nM | 5.36 |
| CHEMBL1235246 | — | Ki | = | 4489.0 | nM | 5.35 |
| CHEMBL5075931 | — | Ki | = | 4878.65 | nM | 5.31 |
| CHEMBL3287574 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL593013 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL1671 | PROPRANOLOL HYDROCHLORIDE | Ki | > | 10000.0 | nM | - |
| CHEMBL2138684 | FROVATRIPTAN SUCCINATE | Ki | > | 10000.0 | nM | - |
| CHEMBL34730 | — | Ki | > | 10000.0 | nM | - |