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Assay Detail

CHEMBL5442202

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
PDSP Secondary Binding target: HTR2C - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
38
Total Activities
38
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Category PDSP assay
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2C (CHEMBL225)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

EUbOPEN Chemogenomics Library - PDSP Secondary Binding
EUbOPEN
(2024)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 38 6.32 8.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL267777 RITANSERIN 2.0 1 8.39
CHEMBL4743049 1 8.03
CHEMBL1489369 1 7.51
CHEMBL83894 FANANSERIN 2.0 1 7.30
CHEMBL2105760 BREXPIPRAZOLE 4.0 1 7.22
CHEMBL74355 VOLINANSERIN 3.0 1 6.94
CHEMBL567303 PALOSURAN 2.0 1 6.86
CHEMBL1516474 TEGASEROD MALEATE 4.0 1 6.85
CHEMBL2107572 TECALCET HYDROCHLORIDE 2.0 1 6.79
CHEMBL5075931 1 6.75
CHEMBL1633 KETOTIFEN FUMARATE 4.0 1 6.71
CHEMBL1200517 DIHYDROERGOTAMINE MESYLATE 4.0 1 6.67
CHEMBL103769 1 6.60
CHEMBL1200492 NEFAZODONE HYDROCHLORIDE 4.0 1 6.60
CHEMBL5089005 1 6.58
CHEMBL259656 1 6.50
CHEMBL531742 1 6.48
CHEMBL540612 1 6.23
CHEMBL1256998 1 6.20
CHEMBL1200776 CINACALCET HYDROCHLORIDE 4.0 1 6.18
CHEMBL281350 SB-224289 1 6.07
CHEMBL214957 1 6.05
CHEMBL1256863 1 5.97
CHEMBL3215877 1 5.93
CHEMBL2024517 CARIPRAZINE HYDROCHLORIDE 4.0 1 5.90
CHEMBL542139 1 5.89
CHEMBL1671 PROPRANOLOL HYDROCHLORIDE 4.0 1 5.86
CHEMBL1215661 PRUVANSERIN 2.0 1 5.83
CHEMBL1095777 INDACATEROL 4.0 1 5.75
CHEMBL405355 NIGULDIPINE 2.0 1 5.73

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL267777 RITANSERIN Ki = 4.09 nM 8.39
CHEMBL4743049 Ki = 9.447 nM 8.03
CHEMBL1489369 Ki = 30.55 nM 7.51
CHEMBL83894 FANANSERIN Ki = 49.98 nM 7.30
CHEMBL2105760 BREXPIPRAZOLE Ki = 60.48 nM 7.22
CHEMBL74355 VOLINANSERIN Ki = 113.89 nM 6.94
CHEMBL567303 PALOSURAN Ki = 138.8 nM 6.86
CHEMBL1516474 TEGASEROD MALEATE Ki = 141.51 nM 6.85
CHEMBL2107572 TECALCET HYDROCHLORIDE Ki = 162.26 nM 6.79
CHEMBL5075931 Ki = 176.64 nM 6.75
CHEMBL1633 KETOTIFEN FUMARATE Ki = 197.38 nM 6.71
CHEMBL1200517 DIHYDROERGOTAMINE MESYLATE Ki = 214.19 nM 6.67
CHEMBL103769 Ki = 251.45 nM 6.60
CHEMBL1200492 NEFAZODONE HYDROCHLORIDE Ki = 253.05 nM 6.60
CHEMBL5089005 Ki = 263.51 nM 6.58
CHEMBL259656 Ki = 319.12 nM 6.50
CHEMBL531742 Ki = 328.25 nM 6.48
CHEMBL540612 Ki = 583.51 nM 6.23
CHEMBL1256998 Ki = 635.26 nM 6.20
CHEMBL1200776 CINACALCET HYDROCHLORIDE Ki = 658.04 nM 6.18
CHEMBL281350 SB-224289 Ki = 859.31 nM 6.07
CHEMBL214957 Ki = 893.2 nM 6.05
CHEMBL1256863 Ki = 1062.92 nM 5.97
CHEMBL3215877 Ki = 1180.05 nM 5.93
CHEMBL2024517 CARIPRAZINE HYDROCHLORIDE Ki = 1253.72 nM 5.90
CHEMBL542139 Ki = 1281.74 nM 5.89
CHEMBL1671 PROPRANOLOL HYDROCHLORIDE Ki = 1373.1 nM 5.86
CHEMBL1215661 PRUVANSERIN Ki = 1472.65 nM 5.83
CHEMBL1095777 INDACATEROL Ki = 1800.32 nM 5.75
CHEMBL405355 NIGULDIPINE Ki = 1854.81 nM 5.73
CHEMBL4164059 PITOLISANT HYDROCHLORIDE Ki = 1987.93 nM 5.70
CHEMBL3342358 Ki = 2215.65 nM 5.65
CHEMBL23327 PURVALANOLA Ki = 3035.29 nM 5.52
CHEMBL1235246 Ki = 3076.45 nM 5.51
CHEMBL191 LOSARTAN Ki = 3493.01 nM 5.46
CHEMBL1263 SALMETEROL Ki = 4092.61 nM 5.39
CHEMBL344159 TOLVAPTAN Ki = 4856.23 nM 5.31
CHEMBL1705 CLONIDINE HYDROCHLORIDE Ki = 6953.44 nM 5.16