Assay Detail
Binding
CHEMBL5442214
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PDSP Secondary Binding target: ADRB2 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 18 | 6.90 | 8.73 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL499 | TIMOLOL | 4.0 | 1 | 8.73 |
| CHEMBL1671 | PROPRANOLOL HYDROCHLORIDE | 4.0 | 1 | 8.64 |
| CHEMBL500 | PINDOLOL | 4.0 | 1 | 8.31 |
| CHEMBL1263 | SALMETEROL | 4.0 | 1 | 8.26 |
| CHEMBL24778 | SILODOSIN | 4.0 | 1 | 7.48 |
| CHEMBL1095777 | INDACATEROL | 4.0 | 1 | 6.97 |
| CHEMBL1374751 | CIMATEROL | 2.0 | 1 | 6.71 |
| CHEMBL1691 | BETAXOLOL HYDROCHLORIDE | 4.0 | 1 | 6.55 |
| CHEMBL5076314 | — | — | 1 | 6.09 |
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 6.01 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | 4.0 | 1 | 5.60 |
| CHEMBL2030155 | — | — | 1 | 5.28 |
| CHEMBL1836317 | — | — | 1 | 5.12 |
| CHEMBL2048820 | — | — | 1 | - |
| CHEMBL1215661 | PRUVANSERIN | 2.0 | 1 | - |
| CHEMBL2181226 | — | — | 1 | - |
| CHEMBL1672558 | — | — | 1 | - |
| CHEMBL1593850 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL499 | TIMOLOL | Ki | = | 1.863 | nM | 8.73 |
| CHEMBL1671 | PROPRANOLOL HYDROCHLORIDE | Ki | = | 2.27 | nM | 8.64 |
| CHEMBL500 | PINDOLOL | Ki | = | 4.91 | nM | 8.31 |
| CHEMBL1263 | SALMETEROL | Ki | = | 5.463 | nM | 8.26 |
| CHEMBL24778 | SILODOSIN | Ki | = | 32.83 | nM | 7.48 |
| CHEMBL1095777 | INDACATEROL | Ki | = | 105.96 | nM | 6.97 |
| CHEMBL1374751 | CIMATEROL | Ki | = | 194.76 | nM | 6.71 |
| CHEMBL1691 | BETAXOLOL HYDROCHLORIDE | Ki | = | 278.42 | nM | 6.55 |
| CHEMBL5076314 | — | Ki | = | 810.5 | nM | 6.09 |
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 968.73 | nM | 6.01 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | Ki | = | 2490.29 | nM | 5.60 |
| CHEMBL2030155 | — | Ki | = | 5205.95 | nM | 5.28 |
| CHEMBL1836317 | — | Ki | = | 7540.5 | nM | 5.12 |
| CHEMBL2048820 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL1215661 | PRUVANSERIN | Ki | > | 10000.0 | nM | - |
| CHEMBL2181226 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL1672558 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL1593850 | — | Ki | > | 10000.0 | nM | - |