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Assay Detail

CHEMBL5657142

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Binding
Inhibition of PI3Kalpha (unknown origin) using PIP2 as substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr in presence of ATP by HTRF assay
17
Total Activities
17
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of GSK2126458, a Highly Potent Inhibitor of PI3K and the Mammalian Target of Rapamycin.
Knight SD, Adams ND, Burgess JL, Chaudhari AM, Darcy MG, Donatelli CA, Luengo JI, Newlander KA, Parrish CA, Ridgers LH, Sarpong MA, Schmidt SJ, Van Aller GS, Carson JD, Diamond MA, Elkins PA, Gardiner CM, Garver E, Gilbert SA, Gontarek RR, Jackson JR, Kershner KL, Luo L, Raha K, Sherk CS, Sung CM, Sutton D, Tummino PJ, Wegrzyn RJ, Auger KR, Dhanak D.
ACS Med Chem Lett (2010) 1 : 39 -43
PubMed 24900173 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 8.08 10.40

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0004992
EFO_TERM EFO_TERM - cancer

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1236962 OMIPALISIB 2.0 1 10.40
CHEMBL5661926 1 10.00
CHEMBL5661866 1 9.00
CHEMBL5661869 1 9.00
CHEMBL3544966 GSK-1059615 1.0 1 8.70
CHEMBL428496 WORTMANNIN 1 8.62
CHEMBL1879463 DACTOLISIB 3.0 1 8.22
CHEMBL1234114 1 8.15
CHEMBL586702 ZSTK-474 2.0 1 8.13
CHEMBL521851 PICTILISIB 2.0 1 8.05
CHEMBL5571102 1 8.00
CHEMBL573339 PI-103 1 7.92
CHEMBL3349370 1 7.41
CHEMBL5661876 1 7.14
CHEMBL5661894 1 6.58
CHEMBL98350 LY-294002 -1.0 1 6.33
CHEMBL5661923 1 5.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1236962 OMIPALISIB IC50 = 0.04 nM 10.40
CHEMBL5661926 IC50 = 0.1 nM 10.00
CHEMBL5661866 IC50 = 1.0 nM 9.00
CHEMBL5661869 IC50 = 1.0 nM 9.00
CHEMBL3544966 GSK-1059615 IC50 = 2.0 nM 8.70
CHEMBL428496 WORTMANNIN IC50 = 2.4 nM 8.62
CHEMBL1879463 DACTOLISIB IC50 = 6.0 nM 8.22
CHEMBL1234114 IC50 = 7.0 nM 8.15
CHEMBL586702 ZSTK-474 IC50 = 7.4 nM 8.13
CHEMBL521851 PICTILISIB IC50 = 9.0 nM 8.05
CHEMBL5571102 IC50 = 10.0 nM 8.00
CHEMBL573339 PI-103 IC50 = 12.0 nM 7.92
CHEMBL3349370 IC50 = 39.0 nM 7.41
CHEMBL5661876 IC50 = 73.0 nM 7.14
CHEMBL5661894 IC50 = 260.0 nM 6.58
CHEMBL98350 LY-294002 IC50 = 470.0 nM 6.33
CHEMBL5661923 IC50 = 1800.0 nM 5.75