Assay Detail
Binding
CHEMBL5735058
Review assay metadata, readout intent, target linkage, and publication context from the same page.
ELISA Assay: VEGF: Four hours later, serial dilutions of 10x compound stocks were made in growth medium from 500xDMSO stocks, and 20 uL of those 10x stocks were added to each well to make final concentrations as follows (uL): 20, 6.67, 2.22, 0.74, 0.25, 0.082, 0.027, 0.009, 0.003, 0.001, and 0. Each concentration had duplicated wells. About 20 hours later, medium was removed by suction and each well was supplied with 180 uL of growth medium. About 20 uL freshly-made 10x compound stocks were added to each well. About 24 hours later, cell culture medium was removed for the determination of VEGFA concentration using an ELISA kit purchased from R&D systems by following the manufacturer's suggested method.
204
Total Activities
66
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Vascular endothelial growth factor A, long form (CHEMBL1783) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Aryl ethers and uses thereof
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 68 | 7.16 | 9.00 |
| kon | 68 | - | - |
| k_off | 68 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5848806 | — | — | 3 | 9.00 |
| CHEMBL4176457 | — | — | 3 | 8.40 |
| CHEMBL5986684 | — | — | 3 | 8.22 |
| CHEMBL5801572 | — | — | 3 | 8.22 |
| CHEMBL5951034 | — | — | 3 | 8.21 |
| CHEMBL4453227 | — | — | 3 | 8.21 |
| CHEMBL6025188 | — | — | 3 | 8.15 |
| CHEMBL5794926 | — | — | 3 | 7.96 |
| CHEMBL4165848 | — | — | 3 | 7.89 |
| CHEMBL5778334 | — | — | 3 | 7.89 |
| CHEMBL5813858 | — | — | 3 | 7.85 |
| CHEMBL4161350 | — | — | 3 | 7.82 |
| CHEMBL5920970 | — | — | 3 | 7.82 |
| CHEMBL5849506 | — | — | 3 | 7.80 |
| CHEMBL6009433 | — | — | 3 | 7.77 |
| CHEMBL4585668 | BELZUTIFAN | 4.0 | 3 | 7.75 |
| CHEMBL6055730 | — | — | 6 | 7.72 |
| CHEMBL4162398 | — | — | 3 | 7.70 |
| CHEMBL5853706 | — | — | 3 | 7.58 |
| CHEMBL5957443 | — | — | 3 | 7.55 |
| CHEMBL5933231 | — | — | 3 | 7.55 |
| CHEMBL5948559 | — | — | 3 | 7.54 |
| CHEMBL4169277 | — | — | 3 | 7.48 |
| CHEMBL6007916 | — | — | 3 | 7.46 |
| CHEMBL4167415 | — | — | 3 | 7.43 |
| CHEMBL5970241 | — | — | 3 | 7.42 |
| CHEMBL5963912 | — | — | 3 | 7.40 |
| CHEMBL4173075 | — | — | 3 | 7.38 |
| CHEMBL5983818 | — | — | 3 | 7.33 |
| CHEMBL6022087 | — | — | 3 | 7.32 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5848806 | — | EC50 | = | 1.0 | nM | 9.00 |
| CHEMBL4176457 | — | EC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5986684 | — | EC50 | = | 6.0 | nM | 8.22 |
| CHEMBL5801572 | — | EC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4453227 | — | EC50 | = | 6.2 | nM | 8.21 |
| CHEMBL5951034 | — | EC50 | = | 6.2 | nM | 8.21 |
| CHEMBL6025188 | — | EC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5794926 | — | EC50 | = | 11.0 | nM | 7.96 |
| CHEMBL4165848 | — | EC50 | = | 13.0 | nM | 7.89 |
| CHEMBL5778334 | — | EC50 | = | 13.0 | nM | 7.89 |
| CHEMBL5813858 | — | EC50 | = | 14.0 | nM | 7.85 |
| CHEMBL5920970 | — | EC50 | = | 15.0 | nM | 7.82 |
| CHEMBL4161350 | — | EC50 | = | 15.0 | nM | 7.82 |
| CHEMBL5849506 | — | EC50 | = | 16.0 | nM | 7.80 |
| CHEMBL6009433 | — | EC50 | = | 17.0 | nM | 7.77 |
| CHEMBL4585668 | BELZUTIFAN | EC50 | = | 18.0 | nM | 7.75 |
| CHEMBL6055730 | — | EC50 | = | 19.2 | nM | 7.72 |
| CHEMBL6055730 | — | EC50 | = | 19.5 | nM | 7.71 |
| CHEMBL4162398 | — | EC50 | = | 20.0 | nM | 7.70 |
| CHEMBL5853706 | — | EC50 | = | 26.0 | nM | 7.58 |
| CHEMBL5957443 | — | EC50 | = | 28.0 | nM | 7.55 |
| CHEMBL5933231 | — | EC50 | = | 28.0 | nM | 7.55 |
| CHEMBL5948559 | — | EC50 | = | 29.0 | nM | 7.54 |
| CHEMBL4169277 | — | EC50 | = | 33.0 | nM | 7.48 |
| CHEMBL6007916 | — | EC50 | = | 35.0 | nM | 7.46 |
| CHEMBL4167415 | — | EC50 | = | 37.0 | nM | 7.43 |
| CHEMBL5970241 | — | EC50 | = | 38.0 | nM | 7.42 |
| CHEMBL5963912 | — | EC50 | = | 40.0 | nM | 7.40 |
| CHEMBL4173075 | — | EC50 | = | 42.0 | nM | 7.38 |
| CHEMBL5983818 | — | EC50 | = | 47.0 | nM | 7.33 |
| CHEMBL6022087 | — | EC50 | = | 48.0 | nM | 7.32 |
| CHEMBL5868442 | — | EC50 | = | 50.0 | nM | 7.30 |
| CHEMBL5960698 | — | EC50 | = | 52.0 | nM | 7.28 |
| CHEMBL5993390 | — | EC50 | = | 62.0 | nM | 7.21 |
| CHEMBL6060195 | — | EC50 | = | 62.0 | nM | 7.21 |
| CHEMBL5776788 | — | EC50 | = | 65.0 | nM | 7.19 |
| CHEMBL5886427 | — | EC50 | = | 64.0 | nM | 7.19 |
| CHEMBL4169671 | — | EC50 | = | 67.0 | nM | 7.17 |
| CHEMBL5828252 | — | EC50 | = | 70.0 | nM | 7.16 |
| CHEMBL6053931 | — | EC50 | = | 69.0 | nM | 7.16 |
| CHEMBL5940475 | — | EC50 | = | 81.0 | nM | 7.09 |
| CHEMBL5980618 | — | EC50 | = | 88.0 | nM | 7.06 |
| CHEMBL5859559 | — | EC50 | = | 130.0 | nM | 6.89 |
| CHEMBL5846164 | — | EC50 | = | 130.0 | nM | 6.89 |
| CHEMBL5785748 | — | EC50 | = | 140.0 | nM | 6.85 |
| CHEMBL5964876 | — | EC50 | = | 140.0 | nM | 6.85 |
| CHEMBL5885510 | — | EC50 | = | 150.0 | nM | 6.82 |
| CHEMBL5940475 | — | EC50 | = | 160.0 | nM | 6.80 |
| CHEMBL4160209 | — | EC50 | = | 160.0 | nM | 6.80 |
| CHEMBL5761841 | — | EC50 | = | 180.0 | nM | 6.75 |