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Assay Detail

CHEMBL650178

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibition of binding to the benzodiazepine receptor in rat cerebral cortical membrane using [3H]diazepam as radioligand
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL1907607)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Synthesis of novel 3-substituted beta-carbolines as benzodiazepine receptor ligands: probing the benzodiazepine receptor pharmacophore.
Allen MS, Hagen TJ, Trudell ML, Codding PW, Skolnick P, Cook JM.
J Med Chem (1988) 31 : 1854 -1861
PubMed 2842507 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.77 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL301605 1 8.40
CHEMBL453066 BETA-CCM 1 8.30
CHEMBL12 DIAZEPAM 4.0 1 8.22
CHEMBL53651 1 8.10
CHEMBL52750 1 7.96
CHEMBL299250 1 7.62
CHEMBL298561 1 7.35
CHEMBL54968 1 6.97
CHEMBL54228 1 6.91
CHEMBL53229 1 6.90
CHEMBL55630 1 6.01
CHEMBL299060 1 5.93
CHEMBL275224 NORHARMANE 1 5.79
CHEMBL53230 1 5.72
CHEMBL299662 1 5.40
CHEMBL416152 1 4.88
CHEMBL54381 1 4.60
CHEMBL12239 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL301605 IC50 = 4.0 nM 8.40
CHEMBL453066 BETA-CCM IC50 = 5.0 nM 8.30
CHEMBL12 DIAZEPAM IC50 = 6.0 nM 8.22
CHEMBL53651 IC50 = 8.0 nM 8.10
CHEMBL52750 IC50 = 11.0 nM 7.96
CHEMBL299250 IC50 = 24.0 nM 7.62
CHEMBL298561 IC50 = 45.0 nM 7.35
CHEMBL54968 IC50 = 106.0 nM 6.97
CHEMBL54228 IC50 = 124.0 nM 6.91
CHEMBL53229 IC50 = 125.0 nM 6.90
CHEMBL55630 IC50 = 980.0 nM 6.01
CHEMBL299060 IC50 = 1163.0 nM 5.93
CHEMBL275224 NORHARMANE IC50 = 1620.0 nM 5.79
CHEMBL53230 IC50 = 1920.0 nM 5.72
CHEMBL299662 IC50 = 4000.0 nM 5.40
CHEMBL416152 IC50 = 13200.0 nM 4.88
CHEMBL54381 IC50 = 25000.0 nM 4.60
CHEMBL12239 IC50 > 50000.0 nM -