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Assay Detail

CHEMBL681129

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Farnesyltransferase in cells, evaluated in a radiotracer assay for farnesyltransferase inhibition (CRAFTI), in cultured H-ras-transformed Rat cells
27
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Protein farnesyltransferase (CHEMBL2095197)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

3-Aminopyrrolidinone farnesyltransferase inhibitors: design of macrocyclic compounds with improved pharmacokinetics and excellent cell potency.
Bell IM, Gallicchio SN, Abrams M, Beese LS, Beshore DC, Bhimnathwala H, Bogusky MJ, Buser CA, Culberson JC, Davide J, Ellis-Hutchings M, Fernandes C, Gibbs JB, Graham SL, Hamilton KA, Hartman GD, Heimbrook DC, Homnick CF, Huber HE, Huff JR, Kassahun K, Koblan KS, Kohl NE, Lobell RB, Lynch JJ, Robinson R, Rodrigues AD, Taylor JS, Walsh ES, Williams TM, Zartman CB.
J Med Chem (2002) 45 : 2388 -2409
PubMed 12036349 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 8.75 10.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL310586 1 10.49
CHEMBL525369 (1R,5R)-30-OXO-19-OXA-2,6,10,12-TETRAAZAHEXACYCLO[18.6.2.1^{2,5}.1^{14,18}.0^{8,12}.0^{23,27}]TRIACONTA-8,10,14(29),15,17,20(28),21,23(27)-OCTAENE-17-CARBONITRILE (STRUCTURAL MIX) 1 10.27
CHEMBL516874 1 10.20
CHEMBL311561 1 9.72
CHEMBL525368 1 9.68
CHEMBL526521 1 9.68
CHEMBL526466 1 9.66
CHEMBL52073 1 9.54
CHEMBL80702 2 9.49
CHEMBL312137 1 9.28
CHEMBL498100 1 9.24
CHEMBL77504 1 9.19
CHEMBL502560 1 9.18
CHEMBL78742 2 9.06
CHEMBL465417 1 8.74
CHEMBL279433 L-778123 FREE BASE 1.0 1 8.44
CHEMBL53821 1 8.42
CHEMBL496879 1 8.36
CHEMBL523080 1 8.17
CHEMBL524082 1 7.89
CHEMBL312121 1 7.41
CHEMBL526656 1 7.39
CHEMBL523570 1 7.03
CHEMBL312034 1 6.70
CHEMBL310943 1 6.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL310586 IC50 = 0.032 nM 10.49
CHEMBL525369 (1R,5R)-30-OXO-19-OXA-2,6,10,12-TETRAAZAHEXACYCLO[18.6.2.1^{2,5}.1^{14,18}.0^{8,12}.0^{23,27}]TRIACONTA-8,10,14(29),15,17,20(28),21,23(27)-OCTAENE-17-CARBONITRILE (STRUCTURAL MIX) IC50 = 0.054 nM 10.27
CHEMBL516874 IC50 = 0.063 nM 10.20
CHEMBL311561 IC50 = 0.19 nM 9.72
CHEMBL525368 IC50 = 0.21 nM 9.68
CHEMBL526521 IC50 = 0.21 nM 9.68
CHEMBL526466 IC50 = 0.22 nM 9.66
CHEMBL52073 IC50 = 0.29 nM 9.54
CHEMBL80702 IC50 = 0.32 nM 9.49
CHEMBL80702 IC50 = 0.44 nM 9.36
CHEMBL312137 IC50 = 0.52 nM 9.28
CHEMBL498100 IC50 = 0.57 nM 9.24
CHEMBL77504 IC50 = 0.64 nM 9.19
CHEMBL502560 IC50 = 0.66 nM 9.18
CHEMBL78742 IC50 = 0.87 nM 9.06
CHEMBL465417 IC50 = 1.8 nM 8.74
CHEMBL279433 L-778123 FREE BASE IC50 = 3.6 nM 8.44
CHEMBL53821 IC50 = 3.8 nM 8.42
CHEMBL496879 IC50 = 4.4 nM 8.36
CHEMBL523080 IC50 = 6.8 nM 8.17
CHEMBL524082 IC50 = 13.0 nM 7.89
CHEMBL312121 IC50 = 39.0 nM 7.41
CHEMBL526656 IC50 = 41.0 nM 7.39
CHEMBL78742 IC50 = 74.0 nM 7.13
CHEMBL523570 IC50 = 93.0 nM 7.03
CHEMBL312034 IC50 = 200.0 nM 6.70
CHEMBL310943 IC50 = 230.0 nM 6.64