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Assay Detail

CHEMBL703402

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity at [3H]CD radiolabeled muscarinic M2 receptor in rat cortex.
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Cortex
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M2 (CHEMBL309)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

The synthesis and biochemical pharmacology of enantiomerically pure methylated oxotremorine derivatives.
Trybulski EJ, Zhang J, Kramss RH, Mangano RM.
J Med Chem (1993) 36 : 3533 -3541
PubMed 8246221 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 7.32 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL7634 OXOTREMORINE 1 9.30
CHEMBL23957 OXOTREMORINE METHIODIDE 1 9.11
CHEMBL310852 1 8.40
CHEMBL14 CARBACHOL 4.0 1 8.40
CHEMBL41779 1 8.20
CHEMBL109815 1 7.82
CHEMBL118292 1 7.64
CHEMBL118700 1 7.26
CHEMBL118240 1 7.25
CHEMBL119615 1 7.15
CHEMBL442206 1 6.58
CHEMBL325473 1 6.35
CHEMBL119985 1 6.20
CHEMBL119508 1 6.01
CHEMBL333107 1 5.82
CHEMBL331556 1 5.60
CHEMBL120706 1 -
CHEMBL118949 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL7634 OXOTREMORINE Ki = 0.5 nM 9.30
CHEMBL23957 OXOTREMORINE METHIODIDE Ki = 0.78 nM 9.11
CHEMBL310852 Ki = 4.0 nM 8.40
CHEMBL14 CARBACHOL Ki = 4.0 nM 8.40
CHEMBL41779 Ki = 6.3 nM 8.20
CHEMBL109815 Ki = 15.0 nM 7.82
CHEMBL118292 Ki = 23.0 nM 7.64
CHEMBL118700 Ki = 55.0 nM 7.26
CHEMBL118240 Ki = 56.0 nM 7.25
CHEMBL119615 Ki = 71.0 nM 7.15
CHEMBL442206 Ki = 260.0 nM 6.58
CHEMBL325473 Ki = 450.0 nM 6.35
CHEMBL119985 Ki = 630.0 nM 6.20
CHEMBL119508 Ki = 980.0 nM 6.01
CHEMBL333107 Ki = 1500.0 nM 5.82
CHEMBL331556 Ki = 2500.0 nM 5.60
CHEMBL120706 Ki > 10000.0 nM -
CHEMBL118949 Ki > 10000.0 nM -