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Assay Detail

CHEMBL744230

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Effective concentration for HMPA (human muscarinic inositol phosphate accumulation) activity measured in CHO cells expressing Muscarinic acetylcholine receptor M1
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type CHO
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M1 (CHEMBL216)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A rationale for the design and synthesis of m1 selective muscarinic agonists.
Tecle H, Mirzadegan T, Moos W, Moreland D, Pavia M, Schwarz R, Davis R
Bioorg Med Chem Lett (1992) 2 : 821 -826
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 5.24 6.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL315676 1 6.05
CHEMBL354738 1 5.72
CHEMBL84893 1 5.48
CHEMBL74300 1 5.46
CHEMBL313918 1 5.33
CHEMBL168561 1 5.30
CHEMBL83074 1 5.29
CHEMBL14 CARBACHOL 4.0 1 5.25
CHEMBL168545 1 5.23
CHEMBL167438 1 4.57
CHEMBL314737 OXADIAZOLE 1 4.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL315676 EC50 = 890.0 nM 6.05
CHEMBL354738 EC50 = 1900.0 nM 5.72
CHEMBL84893 EC50 = 3300.0 nM 5.48
CHEMBL74300 EC50 = 3500.0 nM 5.46
CHEMBL313918 EC50 = 4700.0 nM 5.33
CHEMBL168561 EC50 = 5050.0 nM 5.30
CHEMBL83074 EC50 = 5100.0 nM 5.29
CHEMBL14 CARBACHOL EC50 = 5690.0 nM 5.25
CHEMBL168545 EC50 = 5900.0 nM 5.23
CHEMBL167438 EC50 = 27000.0 nM 4.57
CHEMBL314737 OXADIAZOLE EC50 = 100000.0 nM 4.00