Assay Detail
Binding
CHEMBL744901
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Compound was evaluated for its affinity towards Muscarinic acetylcholine receptor M2, using [3H]- N-methyl-scopolamine, a radioligand displacement assay in rat heart membranes
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Tissue | Heart |
| Subcellular | Membrane |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M2 (CHEMBL309) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Synthesis and biological activity of enantiomers of a conformationally restricted muscarone analog
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 4 | 5.08 | 5.60 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL50898 | — | — | 1 | 5.60 |
| CHEMBL40121 | — | — | 1 | 5.28 |
| CHEMBL550 | PILOCARPINE | 4.0 | 1 | 5.01 |
| CHEMBL50831 | — | — | 1 | 4.43 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL50898 | — | Ki | = | 2500.0 | nM | 5.60 |
| CHEMBL40121 | — | Ki | = | 5300.0 | nM | 5.28 |
| CHEMBL550 | PILOCARPINE | Ki | = | 9800.0 | nM | 5.01 |
| CHEMBL50831 | — | Ki | = | 37000.0 | nM | 4.43 |