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Assay Detail

CHEMBL747492

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Binding
Binding activity against muscarinic acetylcholine receptor M1 in rat brain, using [3H]OXO-M as the radioligand.
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M1 (CHEMBL276)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Functionally selective M1 muscarinic agonists. 3. Side chains and azacycles contributing to functional muscarinic selectivity among pyrazinylazacycles.
Ward JS, Merritt L, Calligaro DO, Bymaster FP, Shannon HE, Sawyer BD, Mitch CH, Deeter JB, Peters SC, Sheardown MJ, Olesen PH, Swedberg MD, Sauerberg P.
J Med Chem (1995) 38 : 3469 -3481
PubMed 7658434 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.90 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL115648 1 9.30
CHEMBL331805 1 9.15
CHEMBL21536 XANOMELINE 4.0 1 8.70
CHEMBL332700 1 8.70
CHEMBL2092949 1 8.60
CHEMBL443282 1 8.40
CHEMBL102560 1 8.40
CHEMBL117666 1 8.35
CHEMBL102588 1 8.34
CHEMBL323719 1 8.30
CHEMBL325964 1 8.25
CHEMBL115473 1 8.22
CHEMBL325225 1 8.05
CHEMBL116462 1 8.00
CHEMBL1788236 1 7.95
CHEMBL117903 1 7.70
CHEMBL105136 1 7.64
CHEMBL115803 1 7.64
CHEMBL117202 1 7.34
CHEMBL327065 1 7.25
CHEMBL117268 1 7.14
CHEMBL7303 ARECOLINE -1.0 1 7.11
CHEMBL115416 1 6.93
CHEMBL117193 1 6.78
CHEMBL117305 1 6.75
CHEMBL74300 1 6.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL115648 IC50 = 0.5 nM 9.30
CHEMBL331805 IC50 = 0.7 nM 9.15
CHEMBL21536 XANOMELINE IC50 = 2.0 nM 8.70
CHEMBL332700 IC50 = 2.0 nM 8.70
CHEMBL2092949 IC50 = 2.5 nM 8.60
CHEMBL443282 IC50 = 4.0 nM 8.40
CHEMBL102560 IC50 = 4.0 nM 8.40
CHEMBL117666 IC50 = 4.5 nM 8.35
CHEMBL102588 IC50 = 4.6 nM 8.34
CHEMBL323719 IC50 = 5.0 nM 8.30
CHEMBL325964 IC50 = 5.6 nM 8.25
CHEMBL115473 IC50 = 6.0 nM 8.22
CHEMBL325225 IC50 = 9.0 nM 8.05
CHEMBL116462 IC50 = 10.0 nM 8.00
CHEMBL1788236 IC50 = 11.3 nM 7.95
CHEMBL117903 IC50 = 20.0 nM 7.70
CHEMBL105136 IC50 = 23.0 nM 7.64
CHEMBL115803 IC50 = 23.0 nM 7.64
CHEMBL117202 IC50 = 46.0 nM 7.34
CHEMBL327065 IC50 = 56.0 nM 7.25
CHEMBL117268 IC50 = 72.0 nM 7.14
CHEMBL7303 ARECOLINE IC50 = 77.0 nM 7.11
CHEMBL115416 IC50 = 117.0 nM 6.93
CHEMBL117193 IC50 = 167.0 nM 6.78
CHEMBL117305 IC50 = 180.0 nM 6.75
CHEMBL74300 IC50 = 355.0 nM 6.45