Assay Detail
Binding
CHEMBL747776
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity against Muscarinic receptor M2 in rat brain using [3H]QNB (quinuclidinyl benzylate) radioligand at a concentration of 0.12 nM
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Tissue | Brain |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M2 (CHEMBL309) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Synthesis and binding affinity of new muscarinic ligands structurally related to oxotremorine
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 5.27 | 5.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL7634 | OXOTREMORINE | — | 1 | 5.70 |
| CHEMBL275104 | — | — | 1 | 5.55 |
| CHEMBL8610 | — | — | 1 | 5.07 |
| CHEMBL274329 | — | — | 1 | 4.76 |
| CHEMBL8510 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL7634 | OXOTREMORINE | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL275104 | — | IC50 | = | 2800.0 | nM | 5.55 |
| CHEMBL8610 | — | IC50 | = | 8600.0 | nM | 5.07 |
| CHEMBL274329 | — | IC50 | = | 17400.0 | nM | 4.76 |
| CHEMBL8510 | — | IC50 | = | 101000.0 | nM | - |