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Assay Detail

CHEMBL747777

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Muscarinic receptor M2 in rat heart using [3H]QNB (quinuclidinyl benzylate) radioligand as a M2 non-selective muscarinic receptor antagonist at a concentration of 0.12 nM
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M2 (CHEMBL309)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and binding affinity of new muscarinic ligands structurally related to oxotremorine
Conti P, Dallanoce C, De Amici M, De Micheli C, Ebert B
Bioorg Med Chem Lett (1997) 7 : 1033 -1036
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.67 7.13

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL274329 1 7.13
CHEMBL7634 OXOTREMORINE 1 7.08
CHEMBL275104 1 6.67
CHEMBL8610 1 6.63
CHEMBL8510 1 5.86

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL274329 IC50 = 74.0 nM 7.13
CHEMBL7634 OXOTREMORINE IC50 = 84.0 nM 7.08
CHEMBL275104 IC50 = 215.0 nM 6.67
CHEMBL8610 IC50 = 233.0 nM 6.63
CHEMBL8510 IC50 = 1380.0 nM 5.86