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Assay Detail

CHEMBL747850

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity was determined against Muscarinic acetylcholine receptor using [3H]QNB as radioligand in rat brain.
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Muscarinic acetylcholine receptor (CHEMBL1907609)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Chloro-substituted, sterically hindered 5,11-dicarbo analogues of clozapine as potential chiral antipsychotic agents.
Davis DA, de Paulis T, Janowsky A, Smith HE.
J Med Chem (1990) 33 : 809 -814
PubMed 1967652 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.73 7.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL73538 1 7.41
CHEMBL42 CLOZAPINE 4.0 1 7.29
CHEMBL415300 ISOCLOZAPINE 1 7.26
CHEMBL91310 1 6.82
CHEMBL355107 1 6.61
CHEMBL73082 1 6.53
CHEMBL72162 1 6.48
CHEMBL88870 1 6.38
CHEMBL328246 1 5.76

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL73538 IC50 = 39.0 nM 7.41
CHEMBL42 CLOZAPINE IC50 = 51.0 nM 7.29
CHEMBL415300 ISOCLOZAPINE IC50 = 55.0 nM 7.26
CHEMBL91310 IC50 = 150.0 nM 6.82
CHEMBL355107 IC50 = 248.0 nM 6.61
CHEMBL73082 IC50 = 297.0 nM 6.53
CHEMBL72162 IC50 = 330.0 nM 6.48
CHEMBL88870 IC50 = 415.0 nM 6.38
CHEMBL328246 IC50 = 1750.0 nM 5.76