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Assay Detail

CHEMBL748279

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Functional
Stimulation of phosphoinositide hydrolysis in A9L cells expressing human m1 receptor
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type A9L
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M1 (CHEMBL216)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of side chains on 1,2,5-thiadiazole-azacycles optimal for muscarinic m1 receptor activation.
Sauerberg P, Jeppesen L, Olesen PH, Sheardown MJ, Fink-Jensen A, Rasmussen T, Rimvall K, Shannon HE, Bymaster FP, DeLapp NW, Calligaro DO, Ward JS, Whitesitt CA, Thomsen C.
Bioorg Med Chem Lett (1998) 8 : 2897 -2902
PubMed 9873644 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 7.21 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL98255 1 9.30
CHEMBL316625 1 9.00
CHEMBL327269 1 9.00
CHEMBL99521 1 7.80
CHEMBL319109 1 7.55
CHEMBL100163 1 7.17
CHEMBL60419 1 7.15
CHEMBL291351 1 6.96
CHEMBL1169506 1 6.80
CHEMBL14 CARBACHOL 4.0 1 5.42
CHEMBL7303 ARECOLINE -1.0 1 5.25
CHEMBL550 PILOCARPINE 4.0 1 5.06
CHEMBL81878 1 -
CHEMBL99884 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL98255 EC50 = 0.5 nM 9.30
CHEMBL316625 EC50 = 1.0 nM 9.00
CHEMBL327269 EC50 = 0.99 nM 9.00
CHEMBL99521 EC50 = 15.7 nM 7.80
CHEMBL319109 EC50 = 28.3 nM 7.55
CHEMBL100163 EC50 = 67.3 nM 7.17
CHEMBL60419 EC50 = 70.5 nM 7.15
CHEMBL291351 EC50 = 110.0 nM 6.96
CHEMBL1169506 EC50 = 159.0 nM 6.80
CHEMBL14 CARBACHOL EC50 = 3811.0 nM 5.42
CHEMBL7303 ARECOLINE EC50 = 5603.0 nM 5.25
CHEMBL550 PILOCARPINE EC50 = 8792.0 nM 5.06
CHEMBL81878 EC50 > 1000.0 nM -
CHEMBL99884 EC50 > 100.0 nM -