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Assay Detail

CHEMBL751538

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]pirenzepine (Pz) from rat hippocampus muscarinic acetylcholine receptor M1
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Hippocampus
Confidence 9 — Direct single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M1 (CHEMBL276)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Novel functional M1 selective muscarinic agonists. 2. Synthesis and structure-activity relationships of 3-pyrazinyl-1,2,5,6-tetrahydro-1-methylpyridines. Construction of a molecular model for the M1 pharmacophore.
Ward JS, Merritt L, Klimkowski VJ, Lamb ML, Mitch CH, Bymaster FP, Sawyer B, Shannon HE, Olesen PH, Honoré T.
J Med Chem (1992) 35 : 4011 -4019
PubMed 1433209 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.57 8.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL130715 1 8.62
CHEMBL327754 1 8.40
CHEMBL131819 TAZOMELINE 2.0 1 8.30
CHEMBL101054 1 8.30
CHEMBL21536 XANOMELINE 4.0 1 8.15
CHEMBL327289 1 7.92
CHEMBL130807 1 7.57
CHEMBL99476 1 7.32
CHEMBL120399 1 7.14
CHEMBL305401 1 6.83
CHEMBL307645 1 6.43
CHEMBL7303 ARECOLINE -1.0 1 5.89
CHEMBL131840 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL130715 IC50 = 2.4 nM 8.62
CHEMBL327754 IC50 = 4.0 nM 8.40
CHEMBL131819 TAZOMELINE IC50 = 5.0 nM 8.30
CHEMBL101054 IC50 = 5.0 nM 8.30
CHEMBL21536 XANOMELINE IC50 = 7.0 nM 8.15
CHEMBL327289 IC50 = 12.0 nM 7.92
CHEMBL130807 IC50 = 27.0 nM 7.57
CHEMBL99476 IC50 = 48.0 nM 7.32
CHEMBL120399 IC50 = 73.0 nM 7.14
CHEMBL305401 IC50 = 148.0 nM 6.83
CHEMBL307645 IC50 = 375.0 nM 6.43
CHEMBL7303 ARECOLINE IC50 = 1300.0 nM 5.89
CHEMBL131840 IC50 > 1000.0 nM -