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Assay Detail

CHEMBL763044

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human PDE4A expressed in Sf9 cells
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Expert

Target

3',5'-cyclic-AMP phosphodiesterase 4A (CHEMBL254)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Hunting the emesis and efficacy targets of PDE4 inhibitors: identification of the photoaffinity probe 8-(3-azidophenyl)-6- [(4-iodo-1H-1-imidazolyl)methyl]quinoline (APIIMQ).
Macdonald D, Perrier H, Liu S, Laliberté F, Rasori R, Robichaud A, Masson P, Huang Z.
J Med Chem (2000) 43 : 3820 -3823
PubMed 11052785 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 8.18 10.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL127944 1 10.15
CHEMBL339083 1 9.70
CHEMBL419236 1 9.40
CHEMBL318502 1 9.22
CHEMBL127994 1 8.57
CHEMBL127201 1 8.48
CHEMBL63 ROLIPRAM 2.0 1 8.40
CHEMBL419416 1 8.05
CHEMBL123576 1 7.82
CHEMBL124575 1 7.55
CHEMBL126587 1 7.46
CHEMBL340379 1 6.60
CHEMBL32442 CDP840 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL127944 IC50 = 0.07 nM 10.15
CHEMBL339083 IC50 = 0.2 nM 9.70
CHEMBL419236 IC50 = 0.4 nM 9.40
CHEMBL318502 IC50 = 0.6 nM 9.22
CHEMBL127994 IC50 = 2.7 nM 8.57
CHEMBL127201 IC50 = 3.3 nM 8.48
CHEMBL63 ROLIPRAM IC50 = 4.0 nM 8.40
CHEMBL419416 IC50 = 9.0 nM 8.05
CHEMBL123576 IC50 = 15.0 nM 7.82
CHEMBL124575 IC50 = 28.0 nM 7.55
CHEMBL126587 IC50 = 35.0 nM 7.46
CHEMBL340379 IC50 = 250.0 nM 6.60
CHEMBL32442 CDP840 IC50 = 10000.0 nM 5.00