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Assay Detail

CHEMBL803322

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of [3H]- AEA metabolism (N-arachidonoylethanolamine) in rat brain homogenates using [3H]anandamide as the labeled substrate.
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Tissue Brain
Confidence 1 — Organism
Curated By Expert

Target

Rattus norvegicus (CHEMBL376)
Type ORGANISM
Organism Rattus norvegicus

Publication

Modifications of the ethanolamine head in N-palmitoylethanolamine: synthesis and evaluation of new agents interfering with the metabolism of anandamide.
Vandevoorde S, Jonsson KO, Fowler CJ, Lambert DM.
J Med Chem (2003) 46 : 1440 -1448
PubMed 12672243 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 5.05 5.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL32433 1 5.60
CHEMBL33355 1 5.58
CHEMBL35286 HEXADECANOIC ACID PROPYLAMIDE 1 5.56
CHEMBL32319 HEXADECANOIC ACID BUTYLAMIDE 1 5.54
CHEMBL32650 HEXADECANOIC ACID ALLYLAMIDE 1 5.47
CHEMBL32886 N-ETHYLPALMITAMIDE 1 5.45
CHEMBL33716 1 5.45
CHEMBL32174 N-(3-HYDROXYPROPYL)PALMITAMIDE 1 5.42
CHEMBL34404 HEXADECANOIC ACID CYCLOPROPYLAMIDE 1 5.35
CHEMBL289007 HEXADECANOIC ACID PHENYLAMIDE 1 5.31
CHEMBL417675 PALMIDROL 3.0 1 5.30
CHEMBL34112 ACETIC ACID PENTADECYLCARBAMOYLMETHYL ESTER 1 5.08
CHEMBL32605 PALMITAMIDE 1 5.01
CHEMBL286321 1 4.99
CHEMBL32827 HEXADECANOIC ACID ISOPROPYLAMIDE 1 4.89
CHEMBL441423 1 4.85
CHEMBL422938 1 4.70
CHEMBL36285 HEXADECANOIC ACID METHYLAMIDE 1 4.57
CHEMBL33245 1 4.40
CHEMBL33143 1 4.27
CHEMBL34340 1 4.15
CHEMBL32504 1 4.07
CHEMBL32472 1 -
CHEMBL34047 HEXADECANOIC ACID DIMETHYLAMIDE 1 -
CHEMBL284042 HEXADECANOIC ACID CYCLOPENTYLAMIDE 1 -
CHEMBL33707 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL32433 IC50 = 2511.89 nM 5.60
CHEMBL33355 IC50 = 2630.27 nM 5.58
CHEMBL35286 HEXADECANOIC ACID PROPYLAMIDE IC50 = 2754.23 nM 5.56
CHEMBL32319 HEXADECANOIC ACID BUTYLAMIDE IC50 = 2884.03 nM 5.54
CHEMBL32650 HEXADECANOIC ACID ALLYLAMIDE IC50 = 3388.44 nM 5.47
CHEMBL32886 N-ETHYLPALMITAMIDE IC50 = 3548.13 nM 5.45
CHEMBL33716 IC50 = 3548.13 nM 5.45
CHEMBL32174 N-(3-HYDROXYPROPYL)PALMITAMIDE IC50 = 3801.89 nM 5.42
CHEMBL34404 HEXADECANOIC ACID CYCLOPROPYLAMIDE IC50 = 4466.84 nM 5.35
CHEMBL289007 HEXADECANOIC ACID PHENYLAMIDE IC50 = 4897.79 nM 5.31
CHEMBL417675 PALMIDROL IC50 = 5011.87 nM 5.30
CHEMBL34112 ACETIC ACID PENTADECYLCARBAMOYLMETHYL ESTER IC50 = 8317.64 nM 5.08
CHEMBL32605 PALMITAMIDE IC50 = 9772.37 nM 5.01
CHEMBL286321 IC50 = 10232.93 nM 4.99
CHEMBL32827 HEXADECANOIC ACID ISOPROPYLAMIDE IC50 = 12882.5 nM 4.89
CHEMBL441423 IC50 = 14125.38 nM 4.85
CHEMBL422938 IC50 = 19952.62 nM 4.70
CHEMBL36285 HEXADECANOIC ACID METHYLAMIDE IC50 = 26915.35 nM 4.57
CHEMBL33245 IC50 = 39810.72 nM 4.40
CHEMBL33143 IC50 = 53703.18 nM 4.27
CHEMBL34340 IC50 = 70794.58 nM 4.15
CHEMBL32504 IC50 = 85113.8 nM 4.07
CHEMBL32472 IC50 = 199526.23 nM -
CHEMBL34047 HEXADECANOIC ACID DIMETHYLAMIDE IC50 = 251188.64 nM -
CHEMBL284042 HEXADECANOIC ACID CYCLOPENTYLAMIDE IC50 = 158489.32 nM -
CHEMBL33707 IC50 = 125892.54 nM -