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Assay Detail

CHEMBL833099

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of HCV RNA replication in a subgenomic bicistronic replicon assay in HB-1 cells
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Hepacivirus hominis
Cell Type HB-1
Confidence 3 — Cell-line / Tissue
Curated By Intermediate

Target

Nucleic Acid (CHEMBL345)
Type NUCLEIC-ACID

Publication

Structure-activity relationship of heterobase-modified 2'-C-methyl ribonucleosides as inhibitors of hepatitis C virus RNA replication.
Eldrup AB, Prhavc M, Brooks J, Bhat B, Prakash TP, Song Q, Bera S, Bhat N, Dande P, Cook PD, Bennett CF, Carroll SS, Ball RG, Bosserman M, Burlein C, Colwell LF, Fay JF, Flores OA, Getty K, LaFemina RL, Leone J, MacCoss M, McMasters DR, Tomassini JE, Von Langen D, Wolanski B, Olsen DB.
J Med Chem (2004) 47 : 5284 -5297
PubMed 15456273 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 5.71 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2023964 1 7.16
CHEMBL364578 1 7.10
CHEMBL2374433 1 6.89
CHEMBL2374434 1 6.62
CHEMBL236207 BETA-2'-C-METHYL-7-DEAZA-ADENOSINE 1 6.60
CHEMBL73809 1 6.58
CHEMBL362765 1 5.51
CHEMBL418899 2'-B-METHYL-GUANOSINE 1 5.46
CHEMBL475504 1 4.47
CHEMBL2374436 1 4.16
CHEMBL2374432 1 4.00
CHEMBL475702 1 4.00
CHEMBL373117 1 -
CHEMBL2374431 1 -
CHEMBL2374435 1 -
CHEMBL2374430 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2023964 EC50 = 70.0 nM 7.16
CHEMBL364578 EC50 = 80.0 nM 7.10
CHEMBL2374433 EC50 = 130.0 nM 6.89
CHEMBL2374434 EC50 = 240.0 nM 6.62
CHEMBL236207 BETA-2'-C-METHYL-7-DEAZA-ADENOSINE EC50 = 250.0 nM 6.60
CHEMBL73809 EC50 = 260.0 nM 6.58
CHEMBL362765 EC50 = 3100.0 nM 5.51
CHEMBL418899 2'-B-METHYL-GUANOSINE EC50 = 3500.0 nM 5.46
CHEMBL475504 EC50 = 34000.0 nM 4.47
CHEMBL2374436 EC50 = 70000.0 nM 4.16
CHEMBL2374432 EC50 = 100000.0 nM 4.00
CHEMBL475702 EC50 = 100000.0 nM 4.00
CHEMBL373117 EC50 > 100000.0 nM -
CHEMBL2374431 EC50 > 100000.0 nM -
CHEMBL2374435 EC50 > 100000.0 nM -
CHEMBL2374430 EC50 > 100000.0 nM -